Automated Proteogenomic Analysis of Porphyromonas gingivalis W83.



Peptide Identification

Number of genomic elements = 1
Name Length
Chromosome Porphyromonas gingivalis W83 {Porphyromonas gingivalis W83}2343476

Number of Spectra: 5721297

Peptide Spectra
IDs on Actual Database 25716 495385
IDs on Decoy Database 1244 3752
False Discovery Rate 0.048 0.008

The above mentioned false discovery rates are computed in the database search using a six-frame translation of the genome and a decoy database of the same size. The false discovery rates, counting only the hits in the coding regions and using decoy database of the size of proteome (more common practise), are lower: 0.001 at the spectrum level and 0.007 at the peptide level.

Access List of Identified Peptides


Protein Identification

Number of annotated ORFs = 1988
Number of proteins confirmed with 2 or more peptides = 1192 | Access List
Number of proteins having only 1 peptide = 179 | Access List


The histogram of number of unique peptides per protein. X axis has the number of unique peptides per protein, Y axis has the frequency (number of proteins). Note that the bins are of size 2, and the plot is truncated at 100 (there may be proteins that have more peptides).



The histogram of percent coverage of the proteins by the identified peptides. X axis has the coverage in percent, Y axis has the frequency (number of proteins). The bins are of size 10 percentage points.




Improving Genome Annotation

Number of peptides within genes: 23808
Number of peptides crossing gene boundaries: 49
Number of peptides outside genes: 1367

Newly Identified Protein Coding Segments:

CoordinatesLength#Peptides#SpectraPossibleExplanation
Seq1:29257-29373 (+1)39157N_Term_Extension of PG_0026
Seq1:237562-237603 (+1)1411Unknown
Seq1:353008-353088 (+1)2711Unknown
Seq1:462376-462390 (+1)511N_Term_Extension of PG_0423
Seq1:486610-486657 (+1)16110Unknown
Seq1:530890-530967 (+1)26519N_Term_Extension of PG_0490
Seq1:715870-715956 (+1)29320Unknown
Seq1:716320-716388 (+1)2322New Gene
Seq1:775186-775368 (+1)61319Unknown
Seq1:957145-957345 (+1)6724Unknown
Seq1:1040158-1040172 (+1)511N_Term_Extension of PG_0978
Seq1:1410625-1411698 (+1)358844634Unknown
Seq1:1501240-1501434 (+1)6511Unknown
Seq1:1625563-1625934 (+1)124415New Gene
Seq1:1626235-1627230 (+1)33214260New Gene
Seq1:1628425-1628577 (+1)5114695N_Term_Extension of PG_1551
Seq1:1637869-1637937 (+1)2323New Gene
Seq1:1654744-1654758 (+1)5142N_Term_Extension of PG_1577
Seq1:1724566-1724598 (+1)1126New Gene
Seq1:30677-30805 (+2)4322Unknown
Seq1:163475-163591 (+2)3923New Gene
Seq1:673025-673102 (+2)2638N_Term_Extension of PG_0620
Seq1:715487-715732 (+2)82655Unknown
Seq1:951437-951535 (+2)33116Unknown
Seq1:1160282-1160338 (+2)19113Unknown
Seq1:1235174-1235749 (+2)19227343Unknown
Seq1:1765631-1766062 (+2)144516Unknown
Seq1:2157704-2157712 (+2)311N_Term_Extension of PG_2060
Seq1:2186660-2186710 (+2)1714Unknown
Seq1:468120-468125 (+3)213N_Term_Extension of PG_0430
Seq1:545436-545474 (+3)1312Unknown
Seq1:844413-844427 (+3)511N_Term_Extension of PG_0793
Seq1:943197-943202 (+3)211N_Term_Extension of PG_0881
Seq1:1080537-1080707 (+3)5711Unknown
Seq1:1089531-1089809 (+3)9316376N_Term_Extension of PG_1028
Seq1:1315341-1315601 (+3)8727Unknown
Seq1:1663020-1663217 (+3)6623Unknown
Seq1:1762278-1762481 (+3)6811Unknown
Seq1:1890129-1890152 (+3)812N_Term_Extension of PG_1797
Seq1:1939890-1941920 (+3)6771264268Unknown
Seq1:2088549-2088599 (+3)1722New Gene
Seq1:2149773-2149775 (+3)118N_Term_Extension of PG_2052
Seq1:2222724-2222756 (+3)1112Unknown
Seq1:2342217-2342489 (+3)91414Unknown
Seq1:2165070-2165246 (-1)5923New Gene
Seq1:2048154-2048306 (-1)51770Unknown
Seq1:1908198-1908236 (-1)1312N_Term_Extension of PG_1812
Seq1:1906848-1907135 (-1)9621130Unknown
Seq1:1883556-1883711 (-1)52219Unknown
Seq1:1545234-1545344 (-1)3724Unknown
Seq1:1228410-1228571 (-1)5415Unknown
Seq1:793197-793223 (-1)923Unknown
Seq1:695934-696869 (-1)31224366Unknown
Seq1:378453-378470 (-1)6112N_Term_Extension of PG_0343
Seq1:254433-254591 (-1)5322Unknown
Seq1:2061419-2061985 (-2)189222235Unknown
Seq1:1851803-1851814 (-2)411N_Term_Extension of PG_1763
Seq1:1831115-1831150 (-2)12112N_Term_Extension of PG_1745
Seq1:1560461-1560535 (-2)2522Unknown
Seq1:1227935-1228369 (-2)1451069Unknown
Seq1:1227335-1227388 (-2)1825Unknown
Seq1:1198811-1198978 (-2)56510N_Term_Extension of PG_1122
Seq1:1198433-1198522 (-2)3014N_Term_Extension of PG_1122
Seq1:1194956-1194988 (-2)11228Unknown
Seq1:1086038-1086364 (-2)10935New Gene
Seq1:941636-941683 (-2)1611N_Term_Extension of PG_0877
Seq1:694865-695887 (-2)341391075New Gene
Seq1:476576-476599 (-2)822Unknown
Seq1:426944-426988 (-2)1522New Gene
Seq1:86210-86245 (-2)1211N_Term_Extension of PG_0071
Seq1:2217541-2217546 (-3)212N_Term_Extension of PG_2108
Seq1:2187118-2187144 (-3)923New Gene
Seq1:2141200-2141406 (-3)6934N_Term_Extension of PG_2042
Seq1:2080105-2080767 (-3)221779New Gene
Seq1:2079583-2079630 (-3)16218Unknown
Seq1:2051851-2051985 (-3)45424New Gene
Seq1:2051473-2051610 (-3)46315Unknown
Seq1:2024227-2024229 (-3)111N_Term_Extension of PG_1937
Seq1:2018446-2018766 (-3)10726Unknown
Seq1:1946698-1946706 (-3)311N_Term_Extension of PG_1847
Seq1:1857493-1857942 (-3)150980New Gene
Seq1:1793920-1793943 (-3)811N_Term_Extension of PG_1705
Seq1:1724107-1724307 (-3)67431Unknown
Seq1:1278253-1279005 (-3)2511288Unknown
Seq1:1151662-1151820 (-3)5323Unknown
Seq1:861856-861888 (-3)11122N_Term_Extension of PG_0804
Seq1:820624-821175 (-3)184981N_Term_Extension of PG_0767
Seq1:795877-796035 (-3)5311Unknown
Seq1:716725-716856 (-3)4422Unknown
Seq1:525415-525474 (-3)2014Unknown
Seq1:281332-281385 (-3)184130N_Term_Extension of PG_0246
Seq1:189181-189183 (-3)113N_Term_Extension of PG_0167
Seq1:35071-35133 (-3)21236New Gene

Each segment represents a putative coding region that is not contained within known ORFs. The number of peptides reported in this table includes only the peptides that lie outside the known ORFs. If the number is 1, it means that there must be another peptide nearby within a known ORF, since we require atleast two peptides for any new segment.



Analyzing Non-tryptic Peptides

Number of peptides with both end non-tryptic = 1576
Number of peptides with one end non-tryptic = 11499

Number of contained peptides = 7724
- Peptides that are contained at their N-terminal side = 5499
- Peptides that are contained at their C-terminal side = 2738

Number of non-Contained peptides (i.e. peptides that are not a subsequence of other peptides) = 5351
Number of non-Covered peptides (non-Contained and located within confirmed proteins) = 4435
Number of non-Covered Ntermini (note that one terminus of a nonCovered peptide may be covered; here we look for cases where N-terminus is not covered) = 2288
Number of non-Covered Ntermini with no upstream coverage in the protein = 240

The plot gives the distribution of residues distances from a non-tryptic end-point to the endpoint in the containing tryptic peptide. X axis shows the distance and Y axis shows the frequency.



Distribution of the N-termini of all non-covered peptides, and of those which also have no upstream coverage. X axis shows the distance of the peptide from N-terminus of the protein, and Y shows has the frequency.




N-Terminal Methionine Cleavage


39 proteins found with N-terminal Methionine Cleavages


Efficiency factor of N-terminal methionine cleavage for each amino acid at second position, as observed in this proteomic analysis and in-vitro analysis of E. coli [Hirel et al. 1989]. The amino acids are ordered in the increasing order of the size of their side chains. Amino acids that occur less than 10 times at the second position are shown with an asterisk.



AminoAcid FrequencyCut FrequencyNoCut CleavageEfficiency HirelEtAlEfficiency
G 2 1 0.666666666666667 0.971
A 19 1 0.95 0.958
P 4 0 1 0.882
S 9 0 1 0.84
T 3 11 0.214285714285714 0.897
V 0 0 NA 0.837
C 0 0 NA 0.71
N 0 27 0 0.164
D 0 6 0 0.161
L 0 3 0 0.163
I 0 9 0 0.184
H 0 2 0 0
Q 0 2 0 0
E 0 16 0 0
F 0 2 0 0
M 0 1 0 0
K 2 21 0.0869565217391304 0
Y 0 2 0 0
W 0 0 NA 0
R 0 0 NA 0

The table reports the N-terminal methionine cleavage efficiency for different amino acids at second position. For each amion acid at second position, the number of proteins with a N-terminal methionine cleavage and with no cleavage are reported, and compared with the efficiency values reported in [Hirel et al. 1989].




Signal Peptides


Venn diagram of all signal peptide predictions on confirmed proteins.



Sequence logo for the amino acid sequences motif of all signal peptides identified by our analysis. Position -1 corresponds to the last residue of the signal peptide.



Number of signal predictions by SignalP and Predisi rejected by us based on observation of peptides upstream of the signal cleavage site.



Unrestricted Modifications Search

Identified 7255 unique modification sites in the organism, at 5% false discovery rate. These are observed by 8254 peptides in the forward database (408 in the shuffled database). Note that two or more different peptide species may correspond to the same modification site.


AminoAcidModificationMassNumPeptidesPeptidesWithUnmodifiedVersionsPossibleAnnotation
+M 16 2069 1908 oxidation M
+C 152 310 294 ?
+W 16 277 267 oxidation W
+N -17 221 182 succinimide formation
+K 127 140 137 ?
+R -70 101 1 ?
+K 128 96 85 lysine adduct
+R -56 95 0 ?
+R -98 78 0 ?
+Q -17 71 51 pyroglutamate formation
+H 12 67 66 ?
+R -86 63 0 ?
+K 14 57 35 methylation
+H 5 54 54 ?
+F 16 51 42 ?
+D -18 48 34 dehydration
+K 28 47 20 dimethylation
+M 32 45 36 double oxidation M
+R -96 44 0 ?
+T -30 44 1 ?
+W 32 44 41 double oxidation W
+K -18 44 24 ?
+V 14 43 3 ?
+H 16 42 42 ?
+W 4 40 38 Oxidation (Trp to kynurenine)
+M 17 40 36 ?
+V 43 36 32 carbamylation
+I -14 36 5 ?
+A 30 35 1 ?
+T 12 34 15 ?
+R 3 33 32 ?
+K 43 31 19 carbamylation K
+H 34 30 27 ?
+A 43 29 26 carbamylation
+G 43 27 24 carbamylation
+W -128 27 22 ?
+G 57 26 19 NT+CAM
+T -14 25 2 ?
+G 128 24 18 lysine adduct
+M -18 24 1 ?
+I 43 24 23 carbamylation
+V -28 24 0 ?
+A 28 23 1 ?
+R 128 23 21 lysine adduct
+L 43 23 21 carbamylation
+E -18 21 14 dehydration
+E 43 21 21 carbamylation
+L 12 20 18 ?
+S 28 20 6 formaldehyde S
+A 16 20 8 ?
+P 12 20 20 ?
+V 12 20 19 ?
+H 57 20 18 CAM
+T -18 20 11 dehydration
+Y 14 19 19 ?
+N -27 19 0 ?
+R -28 19 1 ?
+S 14 18 1 ?
+I 57 17 14 NT+CAM
+C 153 17 16 ?
+K 156 17 16 ?
+V 57 17 15 NT+CAM
+G 41 16 16 ?
+S 27 16 0 ?
+S -16 16 6 ?
+A 22 15 7 Sodium adduct
+L -17 14 10 ?
+L 128 14 9 lysine adduct
+K 42 14 3 acetylation
+N 12 14 13 ?
+A 57 14 10 NT+CAM
+Y 43 13 13 carbamylation
+F 43 13 12 carbamylation
+G 72 13 3 ?
+W 12 12 11 Formaldehyde adduct
+G 58 12 0 ?
+P 43 12 9 carbamylation
+P -10 12 0 ?
+E 128 12 3 lysine adduct
+S 12 12 10 ?
+K 24 12 9 ?
+Y -26 11 0 ?
+E -14 11 2 ?
+T 30 11 1 ?
+A 70 11 11 ?
+N 57 11 10 NT+CAM
+T 43 11 9 carbamylation
+L 57 11 8 NT+CAM
+I 128 11 7 lysine adduct
+H -23 11 6 ?
+N 3 10 10 ?
+F 12 10 9 ?
+E -17 10 8 ?
+N 43 10 10 carbamylation
+V 30 10 0 ?
+I -17 10 7 ?
+D 14 10 0 ?
+K -14 10 0 ?
+P 16 10 1 ?
+M -48 10 6 M+16-64
+Y -16 10 0 ?
+M 19 9 9 ?
+T 28 9 4 formaldehyde T
+H 43 9 9 carbamylation
+G 22 9 4 Sodium adduct
+M 3 9 9 ?
+C 3 9 9 ?
+V -17 9 8 ?
+R -99 9 0 ?
+K 41 9 9 ?
+F 57 9 7 NT+CAM
+C 135 9 9 ?
+D 43 9 9 carbamylation
+C 168 9 9 ?
+G 30 9 0 ?
+G 70 8 8 ?
+I 14 8 6 ?
+Y 12 8 8 ?
+I 18 8 0 ?
+Y 128 8 7 lysine adduct
+V 128 8 6 lysine adduct
+S 3 8 8 ?
+S 10 8 0 ?
+I 12 8 8 ?
+G 12 8 6 ?
+E -45 8 3 ?
+M 43 8 8 carbamylation
+T 13 8 1 ?
+Y -45 8 1 ?
+L -45 8 3 ?
+C 119 7 7 ?
+K 57 7 7 NT+CAM
+Y -17 7 6 ?
+C 16 7 7 ?
+P 57 7 7 NT+CAM
+S -30 7 0 ?
+D -17 7 4 ?
+C 24 7 5 ?
+C -59 7 6 ?
+L 34 7 1 ?
+P 3 7 7 ?
+I 70 7 5 ?
+E 16 7 6 ?
+K 16 7 6 ?
+K 70 7 5 ?
+V 70 7 6 ?
+Y -48 7 0 ?
+D 12 7 7 ?
+E 12 7 6 ?
+A 44 7 1 ?
+S 42 7 2 ?
+S 70 7 5 ?
+S 43 7 7 carbamylation
+I 16 7 5 ?
+L 3 6 5 ?
+L -16 6 1 ?
+F 70 6 6 ?
+R 4 6 0 ?
+D 156 6 2 ?
+L 14 6 3 ?
+H 32 6 6 ?
+E 70 6 6 ?
+D -58 6 1 ?
+A 42 6 1 acetylation
+D 22 6 3 Sodium adduct
+L 70 6 6 ?
+A 17 6 4 ?
+G 42 6 4 acetylation
+A 115 6 3 ?
+P 14 6 4 ?
+Y 70 6 6 ?
+K 12 6 5 ?
+Q 12 6 6 ?
+I -45 6 6 ?
+R -19 6 0 ?
+Y 57 5 5 NT+CAM
+H -45 5 4 ?
+F -34 5 0 ?
+M 12 5 5 ?
+K 38 5 0 Potassium adduct
+G 3 5 4 ?
+F 26 5 5 ?
+E 26 5 5 ?
+E 57 5 2 NT+CAM
+C -77 5 5 ?
+C -103 5 2 ?
+A 31 5 0 ?
+S 128 5 5 lysine adduct
+F -17 5 5 ?
+N 14 5 2 ?
+R -42 5 4 ?
+C 209 5 5 ?
+H 26 5 1 ?
+R -17 5 4 ?
+K 3 5 2 ?
+K -27 5 0 ?
+V 99 5 0 ?
+C 77 5 5 ?
+H 28 5 5 ?
+D -45 5 1 ?
+N 128 5 4 lysine adduct
+D 70 5 5 ?
+T -17 5 4 ?
+L 115 5 4 ?
+G 16 5 4 ?
+A 12 5 5 ?
+E -71 5 5 ?
+S 57 5 3 NT+CAM
+E 152 5 4 ?
+Y 16 5 4 ?
+P 157 5 0 ?
+V -14 5 1 ?
+I 172 4 3 ?
+E 53 4 3 ?
+G 26 4 2 ?
+L 16 4 4 ?
+S 37 4 0 ?
+N 16 4 4 ?
+C 155 4 4 ?
+V 13 4 2 ?
+W 209 4 4 ?
+Q 3 4 4 ?
+M -17 4 1 ?
+G 157 4 1 ?
+D 227 4 3 ?
+A -14 4 0 ?
+L 18 4 2 ?
+C -9 4 4 ?
+T -29 4 0 ?
+V 15 4 2 ?
+L 156 4 2 ?
+E -72 4 1 ?
+Y 115 4 2 ?
+T 172 4 1 ?
+T -32 4 1 ?
+I 34 4 0 ?
+Q 9 4 1 ?
+D 5 4 3 ?
+L 15 4 1 ?
+T 115 4 0 ?
+E -58 4 2 ?
+L -32 4 1 ?
+R -43 4 0 ?
+T -4 4 0 ?
+D 3 4 4 ?
+A 128 4 4 lysine adduct
+M 195 4 4 ?
+S 16 4 4 ?
+E 19 4 3 ?
+G 156 4 3 ?
+T -31 4 0 ?
+I 115 4 2 ?
+W -114 4 2 ?
+C -57 4 4 C-57
+I 3 4 4 ?
+S 73 4 0 ?
+A 156 4 2 ?
+D 152 4 4 ?
+L -14 4 1 ?
+K 226 4 0 ?
+D 16 4 4 ?
+C 7 4 3 ?
+C -73 4 0 ?
+S 26 3 2 ?
+R 52 3 1 ?
+W -112 3 0 ?
+V 115 3 1 ?
+R 88 3 0 ?
+P -14 3 0 ?
+V 16 3 3 ?
+N -13 3 0 ?
+M 168 3 3 ?
+V -35 3 2 ?
+G 214 3 0 ?
+Y -49 3 0 ?
+T 57 3 3 NT+CAM
+I 114 3 0 ?
+G 38 3 1 Potassium adduct
+H 135 3 3 ?
+L 19 3 2 ?
+N 202 3 0 ?
+F 128 3 2 lysine adduct
+I 209 3 2 ?
+L -44 3 1 ?
+W 30 3 3 ?
+R 38 3 1 Potassium adduct
+M 87 3 1 ?
+E 3 3 3 ?
+Y -22 3 1 ?
+K -15 3 1 ?
+I 15 3 2 ?
+H 115 3 2 ?
+H -9 3 0 ?
+G 152 3 3 ?
+K 211 3 0 ?
+R 107 3 1 ?
+D 57 3 3 NT+CAM
+W 13 3 3 ?
+R -27 3 0 ?
+N 5 3 3 ?
+A 26 3 2 ?
+E 13 3 2 ?
+C 121 3 3 ?
+N 41 3 2 ?
+R 55 3 0 R+55
+R -18 3 1 ?
+V 3 3 3 ?
+V 217 3 0 ?
+T 70 3 3 ?
+I 13 3 3 ?
+P 4 3 3 ?
+V -23 3 0 ?
+V 113 3 0 ?
+P -30 3 0 ?
+E 14 3 3 ?
+N 70 3 3 ?
+G 14 3 2 ?
+N 156 3 2 ?
+C 12 3 3 ?
+P 152 3 3 ?
+A 19 3 3 ?
+H 14 3 3 methylation
+W 43 3 3 carbamylation
+R -69 3 0 ?
+S -22 3 0 ?
+G 127 3 2 ?
+F 115 3 1 ?
+D 42 3 1 acetylation
+Q -31 3 0 ?
+E 5 3 3 ?
+C 32 3 3 ?
+F 152 3 2 ?
+S 76 3 1 ?
+I 30 3 0 ?
+C 156 3 1 ?
+T 3 3 3 ?
+N 49 3 0 ?
+C 70 3 3 ?
+T 27 3 0 ?
+R 17 3 3 ?
+L 149 3 2 ?
+I -30 3 0 ?
+Q -9 3 0 ?
+C -3 3 1 ?
+G 73 3 0 ?
+E 4 3 3 ?
+K 34 3 1 ?
+S -18 3 1 dehydration
+D 26 3 2 ?
+L -26 3 2 ?
+I 241 3 3 ?
+W -116 3 1 ?
+I 38 3 2 Potassium adduct
+I -3 3 2 ?
+R -84 3 0 ?
+I 60 3 1 ?
+I -12 3 0 ?
+G 227 3 1 ?
+I 24 3 2 ?
+C -99 3 1 ?
+Y -97 3 2 ?
+M 57 3 3 NT+CAM
+A 11 3 0 ?
+K 26 3 3 ?
+S 156 3 2 ?
+S 22 3 2 Sodium adduct
+I -13 2 0 ?
+A 89 2 1 ?
+Q 16 2 1 ?
+V 4 2 2 ?
+A 3 2 2 ?
+I -47 2 2 ?
+S 60 2 0 ?
+V 112 2 0 ?
+M 70 2 2 ?
+F 3 2 2 ?
+T 46 2 0 ?
+G 13 2 2 ?
+M 14 2 2 ?
+D -16 2 0 ?
+K 58 2 1 ?
+Y -63 2 0 ?
+A 18 2 1 ?
+A 225 2 1 ?
+G 113 2 0 ?
+L 49 2 1 ?
+L 20 2 1 ?
+K -58 2 0 ?
+H 240 2 2 ?
+L -47 2 0 ?
+P 22 2 0 Sodium adduct
+E 82 2 0 ?
+Q -16 2 1 ?
+L 4 2 2 ?
+H -17 2 2 ?
+G 53 2 2 ?
+T 114 2 0 ?
+F 111 2 1 ?
+N -43 2 1 ?
+P -17 2 2 ?
+F 156 2 2 ?
+Q 87 2 1 ?
+E -13 2 0 ?
+S 102 2 0 phosphorylation + sodium
+I 71 2 0 ?
+E 165 2 0 ?
+L -23 2 0 ?
+W 14 2 1 ?
+E -70 2 0 ?
+M 127 2 1 ?
+E -54 2 0 ?
+L 26 2 2 ?
+E 129 2 0 ?
+D 18 2 0 ?
+N 84 2 0 ?
+S 173 2 0 ?
+E -64 2 0 ?
+M -30 2 0 ?
+K 194 2 0 ?
+V -31 2 0 ?
+E 33 2 1 ?
+L 172 2 1 ?
+T -3 2 1 ?
+K -71 2 0 ?
+D 89 2 0 ?
+D 4 2 1 ?
+L 27 2 1 ?
+A 99 2 1 ?
+S 161 2 0 ?
+S 154 2 2 ?
+G 108 2 0 ?
+G 225 2 1 ?
+M 18 2 2 ?
+E 41 2 1 ?
+I -31 2 0 ?
+M 128 2 1 lysine adduct
+A 216 2 0 ?
+R -64 2 0 ?
+T -11 2 1 ?
+S -17 2 0 ?
+I 228 2 1 ?
+L 147 2 0 ?
+L 102 2 1 ?
+C 63 2 2 ?
+T 16 2 1 ?
+S 80 2 1 phosphorylation
+T 20 2 0 ?
+E 115 2 2 ?
+C 151 2 2 ?
+S 77 2 1 ?
+H -24 2 0 ?
+E -56 2 0 ?
+I 109 2 2 ?
+Y 127 2 2 ?
+L 62 2 0 ?
+V 32 2 0 ?
+N 27 2 2 ?
+W -113 2 0 ?
+S 15 2 0 ?
+C 132 2 2 ?
+A 83 2 0 ?
+F -18 2 1 ?
+L 133 2 0 ?
+I -29 2 0 ?
+C -98 2 0 ?
+L 5 2 2 ?
+F -51 2 0 ?
+V -40 2 1 ?
+N -11 2 1 ?
+A 109 2 0 ?
+K -51 2 1 ?
+G 31 2 0 ?
+R -49 2 0 ?
+A 170 2 2 ?
+K -19 2 0 ?
+V -29 2 1 ?
+C 91 2 2 ?
+T 153 2 2 ?
+K 27 2 1 ?
+I -33 2 1 ?
+H 6 2 2 ?
+I -55 2 0 ?
+C -93 2 2 ?
+S -15 2 0 ?
+Q -25 2 1 ?
+E 210 2 1 ?
+P 144 2 0 ?
+M -32 2 1 ?
+E 72 2 1 ?
+C 29 2 2 ?
+N 129 2 2 ?
+A 200 2 1 ?
+Q -18 2 2 ?
+Y -71 2 2 ?
+Y 71 2 1 ?
+A 56 2 0 ?
+K 225 2 0 ?
+S 74 2 0 ?
+T 87 2 0 ?
+W 57 2 1 NT+CAM
+K -59 2 0 ?
+W -71 2 2 ?
+N 2 2 2 ?
+P -16 2 0 ?
+M 20 2 2 ?
+D 199 2 1 ?
+K -47 2 0 ?
+E 212 2 1 ?
+R 127 2 2 ?
+F -73 2 1 ?
+E -43 2 1 ?
+E 99 2 0 ?
+D 115 2 1 ?
+I 90 2 0 ?
+Y -99 2 1 ?
+E 228 2 2 ?
+K 101 2 1 ?
+R -71 2 0 ?
+G 115 2 1 ?
+G 114 2 1 ?
+N -56 2 0 ?
+K 67 2 2 ?
+L -18 2 1 ?
+Q 29 2 0 ?
+P 17 2 1 ?
+C 10 2 2 ?
+V -18 2 2 ?
+P -18 2 1 ?
+F 38 2 0 Potassium adduct
+M 144 2 1 ?
+K -70 2 0 ?
+C 176 2 2 ?
+N -29 2 0 ?
+R -73 2 0 ?
+A -4 2 1 ?
+V 111 2 0 ?
+I -48 2 0 ?
+H 109 2 1 ?
+C -43 2 1 methylation
+K -30 2 0 ?
+F -77 2 1 ?
+C 120 2 2 ?
+T 40 2 1 ?
+H -69 2 2 ?
+A 101 2 1 ?
+A 146 2 0 ?
+H 19 2 1 ?
+W 28 2 2 ?
+L 28 2 2 ?
+L -30 2 1 ?
+I 111 2 1 ?
+I -10 2 1 ?
+Q 115 2 2 ?
+T -10 2 0 ?
+I 29 2 1 ?
+K 30 2 1 ?
+L 24 2 0 ?
+E 166 2 0 ?
+R -10 2 0 ?
+A 84 2 0 ?
+R -89 2 0 ?
+S 201 2 0 ?
+H 111 2 1 ?
+W -127 2 0 ?
+C 145 2 0 ?
+E -16 2 1 ?
+A 60 2 0 ?
+I -19 2 1 ?
+S 225 2 0 ?
+V 48 2 1 ?
+V 72 2 1 ?
+Y -100 2 0 ?
+C 93 2 2 ?
+V -15 2 0 ?
+Y 3 2 2 ?
+P 42 2 1 acetylation
+M -14 2 1 ?
+C 115 2 0 ?
+D 93 2 1 ?
+Q 26 2 0 ?
+C -86 2 0 ?
+L 152 2 0 ?
+V -21 2 0 ?
+A 29 2 1 ?
+K -37 2 1 ?
+Y 97 2 0 ?
+V 153 2 2 ?
+E -46 2 1 ?
+I 97 2 1 ?
+L 119 2 2 ?
+T 183 2 0 ?
+A 240 2 1 ?
+I -26 2 0 ?
+V 158 2 0 ?
+A 41 2 0 ?
+I -32 2 1 ?
+R 10 2 0 ?
+N 22 2 2 Sodium adduct
+M 13 2 1 ?
+A -10 2 0 ?
+R 133 1 1 ?
+D 34 1 1 ?
+K 243 1 1 ?
+N 80 1 0 ?
+D -8 1 0 ?
+R 46 1 0 ?
+S -27 1 0 ?
+V 58 1 1 ?
+S -26 1 1 ?
+R -9 1 0 ?
+K 115 1 1 ?
+A 211 1 1 ?
+L 163 1 0 ?
+C 195 1 1 ?
+F -49 1 0 ?
+F -67 1 0 ?
+S 126 1 1 ?
+F 202 1 1 ?
+F -26 1 0 ?
+V 149 1 1 ?
+G 177 1 0 ?
+D -37 1 0 ?
+K 71 1 1 ?
+N -28 1 0 ?
+P -26 1 0 ?
+L 150 1 0 ?
+V -4 1 0 ?
+K -66 1 1 ?
+M 59 1 1 ?
+A 78 1 0 ?
+H 209 1 1 ?
+S -10 1 0 ?
+I 124 1 1 ?
+T 69 1 0 ?
+E -39 1 0 ?
+P 153 1 0 ?
+R 81 1 0 ?
+K 154 1 0 ?
+R -83 1 0 ?
+H 27 1 1 ?
+R 236 1 0 ?
+D 71 1 0 ?
+Y 157 1 0 ?
+M -33 1 0 ?
+P 201 1 0 ?
+T 155 1 0 ?
+L 88 1 0 ?
+G 216 1 0 ?
+L 100 1 0 ?
+K 60 1 0 ?
+G 187 1 0 ?
+Q 32 1 1 ?
+V 71 1 0 ?
+T 180 1 0 ?
+D -56 1 0 ?
+Y 53 1 1 ?
+C -2 1 0 ?
+E 46 1 1 ?
+L 120 1 0 ?
+L -25 1 1 ?
+H 76 1 0 ?
+K 6 1 0 ?
+L 89 1 1 ?
+G 231 1 0 ?
+S 54 1 0 ?
+T 210 1 0 ?
+L 84 1 1 ?
+M 7 1 0 ?
+F -58 1 0 ?
+V 42 1 0 ?
+N 199 1 0 ?
+P 5 1 1 ?
+K 227 1 0 ?
+I -51 1 0 ?
+C 164 1 1 ?
+N -10 1 1 ?
+P 13 1 1 ?
+V 45 1 0 ?
+S 92 1 0 ?
+I 127 1 1 ?
+A -3 1 1 ?
+R -29 1 1 ?
+N 186 1 1 ?
+E -40 1 0 ?
+P 49 1 0 ?
+S 69 1 0 ?
+V 89 1 1 ?
+E 155 1 0 ?
+V 24 1 0 ?
+K 4 1 0 ?
+N -15 1 0 ?
+L 72 1 0 ?
+P -23 1 1 ?
+V 184 1 0 ?
+M 42 1 1 acetylation
+K 98 1 0 ?
+E 144 1 1 ?
+E 127 1 1 ?
+E -30 1 0 ?
+T 100 1 0 ?
+E 22 1 1 Sodium adduct
+H 33 1 1 ?
+A 126 1 0 ?
+K 22 1 0 Sodium adduct
+A 23 1 0 ?
+G 61 1 0 ?
+Y 75 1 0 ?
+D -29 1 0 ?
+K -44 1 0 ?
+S -9 1 1 ?
+L 230 1 0 ?
+W -117 1 0 ?
+G 33 1 1 ?
+L -52 1 0 ?
+R 113 1 0 ?
+E 27 1 0 ?
+V -16 1 0 ?
+F 209 1 1 ?
+E 116 1 0 ?
+V -42 1 0 ?
+V 162 1 0 ?
+I 78 1 0 ?
+H 18 1 1 ?
+E 8 1 0 ?
+K 196 1 0 ?
+T 55 1 0 ?
+H -80 1 1 ?
+Q 46 1 1 ?
+Q 30 1 0 ?
+L -22 1 0 ?
+E 24 1 1 ?
+Y 121 1 0 ?
+Q 14 1 0 ?
+E 114 1 0 ?
+M 49 1 0 ?
+N 138 1 0 ?
+S 131 1 0 ?
+M -10 1 1 ?
+I 206 1 1 ?
+V 119 1 0 ?
+C 150 1 1 ?
+K 141 1 1 ?
+H 70 1 1 ?
+G 15 1 1 ?
+E 17 1 0 ?
+W 26 1 1 ?
+Y 111 1 0 ?
+G 223 1 1 ?
+S 163 1 0 ?
+S 86 1 1 ?
+D 54 1 1 ?
+I 99 1 0 ?
+N -26 1 0 ?
+E 66 1 0 ?
+M 73 1 1 ?
+A 144 1 1 ?
+E 51 1 0 ?
+R -94 1 0 ?
+K -64 1 0 ?
+E 197 1 0 ?
+F -84 1 0 ?
+V 35 1 0 ?
+G 206 1 0 ?
+V 53 1 1 ?
+A 116 1 0 ?
+C 125 1 0 ?
+R -14 1 1 ?
+L 103 1 0 ?
+L -19 1 0 ?
+R -59 1 0 ?
+F 46 1 0 ?
+C 5 1 1 ?
+F 39 1 0 ?
+P 89 1 1 ?
+G 28 1 1 ?
+G 185 1 0 ?
+I 35 1 0 ?
+D 214 1 1 ?
+R 2 1 1 ?
+S 85 1 0 ?
+A 69 1 1 ?
+H -54 1 1 ?
+P -29 1 0 ?
+V -10 1 0 ?
+N 127 1 1 ?
+P 18 1 1 ?
+C -96 1 0 ?
+Q 228 1 0 ?
+C -51 1 0 ?
+M -59 1 0 ?
+N -47 1 0 ?
+V -22 1 0 ?
+G 172 1 1 ?
+K -53 1 0 ?
+G 11 1 0 ?
+Y -11 1 0 ?
+C 184 1 1 ?
+F 149 1 1 ?
+R -12 1 0 ?
+Q 27 1 1 ?
+Q -44 1 0 ?
+Q 5 1 1 ?
+N 59 1 0 ?
+Q 98 1 1 ?
+L 228 1 1 ?
+G 196 1 0 ?
+V 26 1 0 ?
+K 151 1 0 ?
+A 218 1 0 ?
+R -7 1 0 ?
+D -27 1 0 ?
+V 186 1 1 ?
+C 59 1 1 ?
+G 181 1 0 ?
+L 179 1 0 ?
+D -6 1 0 ?
+S 9 1 0 ?
+T 179 1 0 ?
+R -38 1 0 ?
+L -42 1 0 ?
+V 75 1 1 ?
+E -42 1 0 ?
+L 22 1 1 Sodium adduct
+V 61 1 1 ?
+L 90 1 1 ?
+F 172 1 0 ?
+S 52 1 0 ?
+V 28 1 1 ?
+V 7 1 1 ?
+I 231 1 0 ?
+F 227 1 0 ?
+P 15 1 1 ?
+D 206 1 0 ?
+G 173 1 1 ?
+P 83 1 0 ?
+R 85 1 1 ?
+R 5 1 1 ?
+L 168 1 1 ?
+D -32 1 0 ?
+G 5 1 1 ?
+G 52 1 0 ?
+K 216 1 0 ?
+R -88 1 0 ?
+N 242 1 0 ?
+T 67 1 1 ?
+E 168 1 1 ?
+E 93 1 0 ?
+T -23 1 1 ?
+D 38 1 0 Potassium adduct
+G 238 1 0 ?
+E 142 1 0 ?
+S 111 1 0 ?
+S -29 1 0 ?
+D 59 1 1 ?
+W 144 1 1 ?
+C 14 1 1 ?
+V 27 1 1 ?
+Y 30 1 0 ?
+K 242 1 0 ?
+Q -67 1 0 ?
+P -34 1 0 ?
+K 203 1 0 ?
+A 34 1 0 ?
+Y -67 1 0 ?
+E 249 1 1 ?
+C 100 1 0 ?
+E 132 1 0 ?
+F 106 1 0 ?
+F 41 1 1 ?
+D 190 1 0 ?
+D -21 1 0 ?
+E -68 1 1 ?
+G 48 1 1 ?
+A 125 1 0 ?
+D 53 1 1 ?
+K 182 1 0 ?
+Q 24 1 0 ?
+S -20 1 1 ?
+S 53 1 1 ?
+E -26 1 0 ?
+R 213 1 1 ?
+G 65 1 0 ?
+S -23 1 1 ?
+L 65 1 0 ?
+Y -44 1 0 ?
+K 19 1 1 ?
+H -71 1 1 ?
+Y -50 1 0 ?
+L 46 1 1 ?
+G 68 1 0 ?
+D -44 1 1 ?
+Q -53 1 0 ?
+P -13 1 1 ?
+G 155 1 1 ?
+R -25 1 0 ?
+K 124 1 0 ?
+L 36 1 0 ?
+L 180 1 0 ?
+F -4 1 1 ?
+C -33 1 1 ?
+Q 199 1 0 ?
+Y -77 1 1 ?
+T 149 1 0 ?
+K -69 1 0 ?
+Q -42 1 0 ?
+S 65 1 0 ?
+R -46 1 0 ?
+A 92 1 0 ?
+T 11 1 0 ?
+P 156 1 0 ?
+K 29 1 1 ?
+H -68 1 1 ?
+G 85 1 0 ?
+N 225 1 1 ?
+N 200 1 0 ?
+C -23 1 1 ?
+E 169 1 0 ?
+D 141 1 0 ?
+Q 156 1 0 ?
+I 116 1 0 ?
+Y 152 1 1 ?
+L -36 1 0 ?
+L -28 1 0 ?
+N 40 1 0 ?
+N 86 1 0 ?
+Q 63 1 0 ?
+D 177 1 1 ?
+S 231 1 1 ?
+L -13 1 0 ?
+L 54 1 0 ?
+A 15 1 1 ?
+N 74 1 0 ?
+Y 80 1 1 phosphorylation
+R 180 1 0 ?
+S 20 1 0 ?
+S 17 1 0 ?
+L 241 1 0 ?
+F -85 1 0 ?
+N -30 1 1 ?
+L -12 1 0 ?
+C -11 1 1 ?
+K 129 1 0 ?
+R -87 1 0 ?
+I -11 1 0 ?
+V 5 1 1 ?
+P -24 1 0 ?
+L 56 1 0 ?
+R 222 1 0 ?
+P -9 1 0 ?
+S 124 1 0 ?
+H -41 1 0 ?
+P 197 1 1 ?
+L -56 1 0 ?
+I 212 1 1 ?
+W -87 1 0 ?
+I 182 1 1 ?
+V 88 1 0 ?
+V 94 1 0 ?
+L 148 1 0 ?
+Q -59 1 1 ?
+T 73 1 0 ?
+P 63 1 0 ?
+P 31 1 0 ?
+I 224 1 0 ?
+V 229 1 0 ?
+H 152 1 1 ?
+Y 32 1 0 ?
+S 95 1 1 ?
+M 215 1 0 ?
+P 176 1 1 ?
+A 7 1 0 ?
+D 153 1 1 ?
+T 216 1 0 ?
+D 64 1 0 ?
+G 243 1 1 ?
+K 201 1 0 ?
+R 103 1 0 ?
+V -12 1 0 ?
+D 8 1 1 ?
+F -78 1 0 ?
+Y 17 1 1 ?
+P 154 1 1 ?
+D -24 1 0 ?
+K 11 1 1 ?
+S 46 1 0 ?
+E 204 1 0 ?
+H -25 1 1 ?
+Q -65 1 0 ?
+G 219 1 0 ?
+E 230 1 1 ?
+Y 60 1 0 ?
+R -77 1 0 ?
+E 28 1 0 ?
+R 199 1 0 ?
+V 161 1 0 ?
+Q 81 1 1 ?
+V -27 1 0 ?
+V 100 1 0 ?
+D 15 1 0 ?
+W 103 1 1 ?
+D 120 1 0 ?
+A 62 1 0 ?
+H 117 1 1 ?
+I 160 1 1 ?
+L -46 1 0 ?
+P 102 1 0 ?
+I 217 1 0 ?
+R 153 1 1 ?
+L -15 1 0 ?
+L 224 1 0 ?
+C 180 1 1 ?
+K 214 1 0 ?
+V 87 1 0 ?
+D 40 1 0 ?
+K -60 1 0 ?
+C 40 1 1 ?
+I -4 1 0 ?
+D 211 1 1 ?
+Y -33 1 0 ?
+T 219 1 0 ?
+Q 129 1 1 ?
+M 91 1 0 ?
+E 161 1 0 ?
+V -32 1 1 ?
+K -56 1 0 ?
+V 201 1 0 ?
+S 155 1 0 ?
+N 33 1 1 ?
+A 9 1 1 ?
+Q 43 1 1 carbamylation
+N 19 1 1 ?
+C -100 1 0 ?
+I -44 1 1 ?
+T -19 1 0 ?
+T 168 1 1 ?
+F 94 1 0 ?
+I 72 1 0 ?
+K 64 1 1 ?
+K 2 1 1 ?
+R 226 1 0 ?
+K 86 1 0 ?
+R 7 1 0 ?
+Q 132 1 0 ?
+E -47 1 0 ?
+V 114 1 1 ?
+L 10 1 0 ?
+T 14 1 0 ?
+T 154 1 0 ?
+D 25 1 1 ?
+V 152 1 1 ?
+K 197 1 0 ?
+I 249 1 1 ?
+A 21 1 1 ?
+H 183 1 1 ?
+K 103 1 0 ?
+R -44 1 0 ?
+K 53 1 1 ?
+V 216 1 1 ?
+G 212 1 0 ?
+M 78 1 0 ?
+M 100 1 1 ?
+S 58 1 0 ?
+Q 68 1 0 ?
+E 200 1 0 ?
+K 168 1 1 ?
+Y -56 1 0 ?
+Y -10 1 0 ?
+D 47 1 0 ?
+V 116 1 0 ?
+Y -82 1 0 ?
+C 4