Automated Proteogenomic Analysis of Shewanella frigidimarina.



Peptide Identification

Number of genomic elements = 1
Name Length
gi|114332481|gb|CP000447.1| Shewanella frigidimarina NCIMB 400, complete genome4845257

Number of Spectra: 944052

Peptide Spectra
IDs on Actual Database 27605 191516
IDs on Decoy Database 1290 1893
False Discovery Rate 0.047 0.010

The above mentioned false discovery rates are computed in the database search using a six-frame translation of the genome and a decoy database of the same size. The false discovery rates, counting only the hits in the coding regions and using decoy database of the size of proteome (more common practise), are lower: 0.001 at the spectrum level and 0.007 at the peptide level.

Access List of Identified Peptides


Protein Identification

Number of annotated ORFs = 4029
Number of proteins confirmed with 2 or more peptides = 1854 | Access List
Number of proteins having only 1 peptide = 512 | Access List


The histogram of number of unique peptides per protein. X axis has the number of unique peptides per protein, Y axis has the frequency (number of proteins). Note that the bins are of size 2, and the plot is truncated at 100 (there may be proteins that have more peptides).



The histogram of percent coverage of the proteins by the identified peptides. X axis has the coverage in percent, Y axis has the frequency (number of proteins). The bins are of size 10 percentage points.




Improving Genome Annotation

Number of peptides within genes: 24445
Number of peptides crossing gene boundaries: 12

Newly Identified Protein Coding Segments:

CoordinatesLength#Peptides#SpectraPossibleExplanation
Seq1:181288-181308 (+1)713N_Term_Extension of Sfri_0170
Seq1:4193713-4193904 (+1)6411Unknown
Seq1:4844467-4844526 (+1)2022New Gene
Seq1:675944-676009 (+2)2235Unknown
Seq1:1154315-1154341 (+2)912Unknown
Seq1:1223393-1223464 (+2)2411N_Term_Extension of Sfri_1039
Seq1:1605029-1605046 (+2)611N_Term_Extension of Sfri_1349
Seq1:2867714-2867752 (+2)1311Unknown
Seq1:4113425-4113727 (+2)10137Unknown
Seq1:676062-676079 (+3)611N_Term_Extension of Sfri_0582
Seq1:1397655-1397777 (+3)4129Unknown
Seq1:2602266-2602358 (+3)3122Unknown
Seq1:3950462-3950467 (-2)212N_Term_Extension of Sfri_3304
Seq1:2929457-2929468 (-2)411N_Term_Extension of Sfri_2461
Seq1:2399531-2399740 (-2)70759N_Term_Extension of Sfri_2027
Seq1:1704638-1704649 (-2)411N_Term_Extension of Sfri_1429
Seq1:1305599-1305613 (-2)511Unknown
Seq1:3943207-3943278 (-3)2412N_Term_Extension of Sfri_3298
Seq1:1907254-1907382 (-3)4325Unknown
Seq1:244567-244644 (-3)2611Unknown

Each segment represents a putative coding region that is not contained within known ORFs. The number of peptides reported in this table includes only the peptides that lie outside the known ORFs. If the number is 1, it means that there must be another peptide nearby within a known ORF, since we require atleast two peptides for any new segment.



Analyzing Non-tryptic Peptides

Number of peptides with both end non-tryptic = 700
Number of peptides with one end non-tryptic = 7114

Number of contained peptides = 5250
- Peptides that are contained at their N-terminal side = 3127
- Peptides that are contained at their C-terminal side = 2269

Number of non-Contained peptides (i.e. peptides that are not a subsequence of other peptides) = 2564
Number of non-Covered peptides (non-Contained and located within confirmed proteins) = 1412
Number of non-Covered Ntermini (note that one terminus of a nonCovered peptide may be covered; here we look for cases where N-terminus is not covered) = 473
Number of non-Covered Ntermini with no upstream coverage in the protein = 315

The plot gives the distribution of residues distances from a non-tryptic end-point to the endpoint in the containing tryptic peptide. X axis shows the distance and Y axis shows the frequency.



Distribution of the N-termini of all non-covered peptides, and of those which also have no upstream coverage. X axis shows the distance of the peptide from N-terminus of the protein, and Y shows has the frequency.




N-Terminal Methionine Cleavage


210 proteins found with N-terminal Methionine Cleavages


Efficiency factor of N-terminal methionine cleavage for each amino acid at second position, as observed in this proteomic analysis and in-vitro analysis of E. coli [Hirel et al. 1989]. The amino acids are ordered in the increasing order of the size of their side chains. Amino acids that occur less than 10 times at the second position are shown with an asterisk.



AminoAcid FrequencyCut FrequencyNoCut CleavageEfficiency HirelEtAlEfficiency
G 6 0 1 0.971
A 53 0 1 0.958
P 14 0 1 0.882
S 102 8 0.927272727272727 0.84
T 32 25 0.56140350877193 0.897
V 0 8 0 0.837
C 0 0 NA 0.71
N 2 35 0.0540540540540541 0.164
D 1 12 0.0769230769230769 0.161
L 0 17 0 0.163
I 0 19 0 0.184
H 0 14 0 0
Q 0 22 0 0
E 0 7 0 0
F 0 12 0 0
M 0 1 0 0
K 0 53 0 0
Y 0 2 0 0
W 0 2 0 0
R 0 4 0 0

The table reports the N-terminal methionine cleavage efficiency for different amino acids at second position. For each amion acid at second position, the number of proteins with a N-terminal methionine cleavage and with no cleavage are reported, and compared with the efficiency values reported in [Hirel et al. 1989].




Signal Peptides


Venn diagram of all signal peptide predictions on confirmed proteins.



Sequence logo for the amino acid sequences motif of all signal peptides identified by our analysis. Position -1 corresponds to the last residue of the signal peptide.



Number of signal predictions by SignalP and Predisi rejected by us based on observation of peptides upstream of the signal cleavage site.



Unrestricted Modifications Search

Identified 7787 unique modification sites in the organism, at 5% false discovery rate. These are observed by 8321 peptides in the forward database (420 in the shuffled database). Note that two or more different peptide species may correspond to the same modification site.


AminoAcidModificationMassNumPeptidesPeptidesWithUnmodifiedVersionsPossibleAnnotation
+M 16 1943 1673 oxidation M
+Q -17 380 262 pyroglutamate formation
+S 28 355 306 formaldehyde S
+W 32 273 250 double oxidation W
+N -17 248 180 succinimide formation
+T 28 206 175 formaldehyde T
+W 4 161 150 Oxidation (Trp to kynurenine)
+C 248 148 8 ?
+M 32 127 88 double oxidation M
+T -18 122 82 dehydration
+D -18 111 61 dehydration
+L 22 101 79 Sodium adduct
+K 28 101 91 dimethylation
+K 43 98 64 carbamylation K
+G 22 85 74 Sodium adduct
+C -57 78 3 C-57
+H 28 69 67 ?
+C 62 61 4 ?
+L -17 60 50 ?
+A 22 59 51 Sodium adduct
+E 22 56 42 Sodium adduct
+Y 14 51 50 ?
+D 22 50 44 Sodium adduct
+W 16 42 41 oxidation W
+W 48 34 34 ?
+Y 16 34 33 ?
+E -18 33 15 dehydration
+V 28 33 30 ?
+A 43 32 18 carbamylation
+L 28 28 27 ?
+V -18 27 14 ?
+C 191 26 4 ?
+L -18 23 15 ?
+V -17 22 17 ?
+K 14 22 11 methylation
+V 22 21 20 Sodium adduct
+I 28 20 20 ?
+M -48 20 10 M+16-64
+A 28 19 19 ?
+E 28 19 19 ?
+C -84 18 0 ?
+E 38 17 7 Potassium adduct
+W -128 15 12 ?
+R 28 15 15 ?
+N 3 14 13 ?
+D 53 14 12 ?
+M 19 13 13 ?
+D -17 13 10 ?
+D 28 13 13 ?
+C 63 13 0 ?
+M 48 13 11 ?
+Q 28 13 13 ?
+K -18 13 11 ?
+N 22 13 13 Sodium adduct
+L 14 12 5 ?
+Y 15 12 12 ?
+C -32 12 2 ?
+L 53 12 10 ?
+G 43 11 8 carbamylation
+Q -18 11 4 ?
+S 43 11 8 carbamylation
+S -18 11 4 dehydration
+Q 14 10 2 ?
+C -9 10 3 ?
+I -17 10 7 ?
+I 53 9 6 ?
+G 28 9 9 ?
+L 128 9 5 lysine adduct
+D 38 9 3 Potassium adduct
+S 53 9 9 ?
+I -14 9 8 ?
+N 28 9 9 ?
+L 3 8 8 ?
+I 14 8 5 ?
+Q 3 8 6 ?
+A 16 8 7 ?
+E 3 8 8 ?
+Y 32 8 7 ?
+M -32 8 6 ?
+V 43 8 7 carbamylation
+L 38 8 2 Potassium adduct
+Y -99 8 5 ?
+Q 22 8 6 Sodium adduct
+F 16 8 8 ?
+D 14 8 6 ?
+I 16 8 8 ?
+C 33 7 4 ?
+E -14 7 4 ?
+L 18 7 4 ?
+I -18 7 4 ?
+M 44 7 7 ?
+K 156 7 7 ?
+M -16 7 7 ?
+M 17 7 4 ?
+W 12 6 5 Formaldehyde adduct
+A 3 6 6 ?
+E 53 6 6 ?
+R 16 6 0 ?
+L 16 6 5 ?
+G 53 6 5 ?
+D 248 6 0 ?
+A 38 6 4 Potassium adduct
+E 43 6 4 carbamylation
+V 128 6 3 lysine adduct
+F 28 6 6 ?
+M 3 6 6 ?
+D 154 6 5 ?
+C 77 6 1 ?
+G 248 6 0 ?
+G 14 6 5 ?
+A 128 6 3 lysine adduct
+L 43 6 3 carbamylation
+C 249 6 0 ?
+M -50 6 6 ?
+D 43 6 2 carbamylation
+M 28 5 5 ?
+V 16 5 4 ?
+G 3 5 5 ?
+V 53 5 4 ?
+I 32 5 4 ?
+D 13 5 0 ?
+A 58 5 2 ?
+F 32 5 4 ?
+L -12 5 1 ?
+C 40 5 0 ?
+S 3 5 4 ?
+N -18 5 2 ?
+C 218 5 2 ?
+C 12 5 0 ?
+T -17 5 4 ?
+T 43 5 1 carbamylation
+M 43 5 4 carbamylation
+N 38 5 3 Potassium adduct
+D -3 5 3 ?
+P 16 5 4 ?
+S 14 5 5 ?
+I 22 5 5 Sodium adduct
+D 56 5 4 ?
+F 3 4 2 ?
+D -25 4 1 ?
+T 19 4 1 ?
+K 131 4 2 ?
+A -3 4 1 ?
+I 48 4 2 ?
+V 14 4 2 ?
+E -21 4 2 ?
+L 12 4 2 ?
+E 17 4 2 ?
+K 38 4 1 Potassium adduct
+L -49 4 1 ?
+F 53 4 3 ?
+A 53 4 4 ?
+V 248 4 1 ?
+A 248 4 0 ?
+A 15 4 2 ?
+V 54 4 2 ?
+E -60 4 1 ?
+T 14 4 2 ?
+I 248 4 1 ?
+V 3 4 2 ?
+L -32 4 1 ?
+W 15 4 4 ?
+L 248 4 1 ?
+G 12 4 1 ?
+Y -101 4 4 ?
+L 70 4 1 ?
+E -62 4 0 ?
+A -13 4 2 ?
+A 88 4 0 ?
+D 3 4 4 ?
+E 10 4 2 ?
+G 51 4 2 ?
+L 33 4 0 ?
+C 93 4 2 ?
+W -116 4 0 ?
+C 7 4 1 ?
+F 154 4 4 ?
+I 49 3 2 ?
+L 114 3 0 ?
+L 100 3 1 ?
+E 104 3 1 ?
+A 104 3 0 ?
+V 77 3 1 ?
+T 42 3 0 acetylation
+V 19 3 1 ?
+W 14 3 3 ?
+G 15 3 0 ?
+G 35 3 2 ?
+M 127 3 0 ?
+C 125 3 2 ?
+G 55 3 2 ?
+C 44 3 1 ?
+Q -71 3 1 ?
+A -14 3 2 ?
+L -42 3 0 ?
+A 35 3 1 ?
+A 154 3 3 ?
+G 38 3 2 Potassium adduct
+L 19 3 2 ?
+G 5 3 2 ?
+V 27 3 1 ?
+D 77 3 2 ?
+I -31 3 0 ?
+G 48 3 2 ?
+A 72 3 0 ?
+E 52 3 1 ?
+H -71 3 3 ?
+T 16 3 3 ?
+I -16 3 2 ?
+L 107 3 3 ?
+R 38 3 0 Potassium adduct
+T -41 3 0 ?
+I 51 3 0 ?
+T -21 3 0 ?
+L -13 3 3 ?
+Y 154 3 3 ?
+L -56 3 1 ?
+A 7 3 0 ?
+T 48 3 1 ?
+R -98 3 0 ?
+V 15 3 1 ?
+H 55 3 2 ?
+L 64 3 0 ?
+D -14 3 2 ?
+Y -31 3 0 ?
+D 57 3 0 NT+CAM
+E -17 3 3 ?
+A 62 3 1 ?
+A 13 3 2 ?
+S -15 3 2 ?
+V -32 3 2 ?
+C -100 3 0 ?
+L 29 3 2 ?
+L -9 3 2 ?
+E 16 3 3 ?
+Y 71 3 3 ?
+K 16 3 2 ?
+S 5 3 3 ?
+E -51 3 0 ?
+I 12 3 0 ?
+L 11 3 0 ?
+N 57 3 2 NT+CAM
+H 16 3 3 ?
+T 45 3 0 ?
+V 17 3 1 ?
+G 114 3 0 ?
+D 19 3 2 ?
+F 43 3 3 carbamylation
+D 215 3 0 ?
+R 124 3 0 ?
+K -70 3 1 ?
+L 85 3 0 ?
+I 10 3 1 ?
+D 98 3 1 ?
+L 48 3 0 ?
+C -43 3 0 methylation
+R 14 3 2 ?
+C 109 3 2 ?
+P 14 3 3 ?
+C 120 3 0 ?
+D 95 3 1 ?
+L 35 3 1 ?
+V 33 3 1 ?
+L 57 3 1 NT+CAM
+I 3 3 3 ?
+A 57 3 0 NT+CAM
+R 195 3 0 ?
+C -3 3 0 ?
+K -22 3 1 ?
+L 108 3 1 ?
+V 97 3 0 ?
+T 80 3 0 phosphorylation
+L 112 3 1 ?
+V 8 3 0 ?
+L 37 3 0 ?
+V -14 3 2 ?
+I 190 2 0 ?
+T 5 2 2 ?
+L -16 2 0 ?
+T 62 2 1 ?
+F 50 2 2 ?
+M 59 2 2 ?
+A 78 2 1 ?
+P 248 2 0 ?
+E 29 2 1 ?
+R 35 2 0 ?
+I 105 2 0 ?
+K 17 2 2 ?
+D 71 2 0 ?
+E 100 2 1 ?
+R -67 2 0 ?
+M -69 2 1 ?
+T 248 2 0 ?
+A 18 2 0 ?
+M -35 2 1 ?
+Q 32 2 0 ?
+D -56 2 0 ?
+E 46 2 0 ?
+K 57 2 2 NT+CAM
+L 84 2 1 ?
+L 51 2 0 ?
+I -51 2 0 ?
+L -47 2 0 ?
+V 218 2 2 ?
+M 122 2 1 ?
+E 109 2 1 ?
+L 72 2 0 ?
+E -66 2 0 ?
+I -25 2 1 ?
+F -32 2 0 ?
+D -29 2 1 ?
+Q -11 2 1 ?
+Y -17 2 2 ?
+T -44 2 1 ?
+L -52 2 1 ?
+D 69 2 2 ?
+L -33 2 1 ?
+E -13 2 0 ?
+L -29 2 0 ?
+N 54 2 1 ?
+D 87 2 2 ?
+E 114 2 1 ?
+E 215 2 1 ?
+E -70 2 0 ?
+G 223 2 1 ?
+D 54 2 2 ?
+V 35 2 0 ?
+L 86 2 0 ?
+A 180 2 0 ?
+V 199 2 1 ?
+Q 55 2 1 ?
+C -31 2 0 ?
+C -83 2 0 ?
+C 162 2 2 ?
+N -31 2 1 ?
+Y 28 2 2 ?
+C 143 2 0 ?
+I -40 2 0 ?
+D -12 2 0 ?
+E 49 2 2 ?
+E -42 2 0 ?
+K -71 2 0 ?
+L 40 2 1 ?
+A 99 2 2 ?
+D -32 2 1 ?
+T -23 2 0 ?
+L -41 2 1 ?
+I -36 2 1 ?
+D 31 2 0 ?
+H -22 2 1 ?
+A 216 2 2 ?
+K -23 2 0 ?
+T 201 2 0 ?
+A 224 2 0 ?
+N -50 2 0 ?
+K 37 2 0 ?
+C 123 2 0 ?
+K 19 2 2 ?
+S 80 2 1 phosphorylation
+Q -53 2 0 ?
+T 140 2 0 ?
+C 94 2 2 ?
+T 11 2 0 ?
+W 49 2 2 ?
+W 30 2 2 ?
+N 200 2 1 ?
+M -43 2 0 ?
+I 121 2 0 ?
+L 8 2 0 ?
+W -113 2 2 ?
+I 143 2 0 ?
+G 29 2 2 ?
+D 94 2 0 ?
+V 90 2 0 ?
+T -30 2 2 ?
+L 133 2 1 ?
+K 122 2 1 ?
+A 2 2 2 ?
+Q 82 2 0 ?
+K 205 2 1 ?
+L 175 2 0 ?
+Y -98 2 1 ?
+C -59 2 1 ?
+E 135 2 0 ?
+L 195 2 0 ?
+F -78 2 1 ?
+Q 11 2 2 ?
+R -66 2 0 ?
+L 44 2 1 ?
+Q 81 2 0 ?
+Q -62 2 1 ?
+D 15 2 2 ?
+A 48 2 2 ?
+G 83 2 0 ?
+L -15 2 1 ?
+V 87 2 1 ?
+L 186 2 0 ?
+D -53 2 0 ?
+V 40 2 0 ?
+P 3 2 2 ?
+Q 52 2 0 ?
+N 5 2 0 ?
+A 9 2 2 ?
+Q 43 2 2 carbamylation
+N 19 2 1 ?
+T -43 2 0 ?
+G 188 2 0 ?
+I -44 2 1 ?
+C 209 2 2 ?
+L 131 2 0 ?
+E -47 2 1 ?
+A 42 2 0 acetylation
+R -55 2 0 ?
+L 15 2 2 ?
+G 89 2 0 ?
+G 200 2 0 ?
+K 48 2 2 ?
+I 2 2 2 ?
+D 10 2 1 ?
+P 72 2 2 ?
+N 159 2 0 ?
+Q -22 2 0 ?
+K -52 2 0 ?
+M 20 2 2 ?
+E 128 2 2 lysine adduct
+A 40 2 0 ?
+E -31 2 0 ?
+H -11 2 1 ?
+E -48 2 0 ?
+S 27 2 2 ?
+Q 15 2 1 ?
+D 83 2 1 ?
+V 34 2 0 ?
+F 64 2 2 ?
+F 22 2 2 Sodium adduct
+F -71 2 2 ?
+G 44 2 2 ?
+E 69 2 0 ?
+I 198 2 1 ?
+L 76 2 1 ?
+N 132 2 1 ?
+S 129 2 1 ?
+V 56 2 1 ?
+C -66 2 0 ?
+N -56 2 0 ?
+I 31 2 2 ?
+E 35 2 1 ?
+W -13 2 2 ?
+A 19 2 1 ?
+K 67 2 0 ?
+N 89 2 0 ?
+K -65 2 0 ?
+E 92 2 1 ?
+T -25 2 0 ?
+Q 174 2 1 ?
+A 14 2 1 ?
+S 55 2 1 ?
+I 21 2 1 ?
+A 213 2 2 ?
+R -78 2 0 ?
+Q -70 2 1 ?
+E 54 2 2 ?
+P -18 2 2 ?
+S 249 2 0 ?
+Q 4 2 2 ?
+S 248 2 0 ?
+P 48 2 2 ?
+L 17 2 0 ?
+A 95 2 0 ?
+E -29 2 0 ?
+P 32 2 1 ?
+I 9 2 0 ?
+N 25 2 0 ?
+S 68 2 0 ?
+H 12 2 1 ?
+D 42 2 0 acetylation
+G 210 2 0 ?
+I -34 2 0 ?
+H 50 2 0 ?
+F -37 2 1 ?
+K 147 2 2 ?
+E 12 2 1 ?
+C 153 2 1 ?
+L 68 2 0 ?
+Y -64 2 1 ?
+G 16 2 1 ?
+T 8 2 2 ?
+Q -31 2 2 ?
+D 29 2 2 ?
+L 25 2 0 ?
+G 4 2 0 ?
+V 83 2 1 ?
+K -63 2 0 ?
+V 25 2 0 ?
+S 16 2 2 ?
+E 50 2 0 ?
+V 50 2 1 ?
+N -32 2 1 ?
+L 104 2 1 ?
+M 65 2 1 ?
+S 12 2 1 ?
+I 19 2 2 ?
+G 57 2 2 NT+CAM
+L -43 2 1 ?
+D 48 2 1 ?
+R -26 2 2 ?
+I 30 2 1 ?
+L -3 2 2 ?
+W 28 2 2 ?
+I 115 2 0 ?
+Y -14 2 1 ?
+R -86 2 0 ?
+F -86 2 0 ?
+W -114 2 1 ?
+F -39 2 0 ?
+I 46 2 0 ?
+P 71 2 1 ?
+S 57 2 0 NT+CAM
+A 84 2 1 ?
+I 33 2 0 ?
+I 69 2 0 ?
+T -38 2 0 ?
+E -69 2 1 ?
+A 60 2 1 ?
+Q -30 2 2 ?
+D 62 2 0 ?
+K 146 2 1 ?
+E 4 2 2 ?
+I -45 2 0 ?
+L -45 2 1 ?
+A 87 2 1 ?
+C 61 2 0 ?
+D 225 2 0 ?
+K -16 2 2 ?
+E -10 2 1 ?
+Q -29 2 1 ?
+I 128 2 2 lysine adduct
+Q 53 2 2 ?
+I 38 2 2 Potassium adduct
+G 249 2 0 ?
+I -3 2 2 ?
+P 62 2 0 ?
+L 189 2 1 ?
+D 128 2 1 lysine adduct
+Q 95 2 0 ?
+A 29 2 2 ?
+L -14 2 1 ?
+I 25 2 0 ?
+T 91 2 1 ?
+Y 126 2 2 ?
+D 16 2 1 ?
+Y -70 2 0 ?
+H 125 2 0 ?
+K 128 2 1 lysine adduct
+K -50 2 1 ?
+T 129 2 0 ?
+D -9 2 1 ?
+C 190 2 0 ?
+M -31 2 1 ?
+A 27 2 1 ?
+F 42 2 1 ?
+P 209 2 1 ?
+A 71 2 1 ?
+K -17 2 2 ?
+M 13 2 2 ?
+P 37 2 1 ?
+R 133 1 0 ?
+P 179 1 0 ?
+D -8 1 1 ?
+R 46 1 0 ?
+M -73 1 0 ?
+Q 16 1 0 ?
+V 58 1 0 ?
+G 240 1 0 ?
+S -26 1 0 ?
+W 74 1 1 ?
+E -5 1 0 ?
+Y 5 1 1 ?
+T 173 1 1 ?
+N -54 1 0 ?
+S 88 1 1 ?
+D -42 1 0 ?
+I 63 1 0 ?
+V 4 1 1 ?
+N 23 1 1 ?
+F -15 1 1 ?
+F -67 1 0 ?
+S 126 1 0 ?
+S 26 1 1 ?
+S 148 1 0 ?
+Y -78 1 0 ?
+W -3 1 1 ?
+A 141 1 0 ?
+E 61 1 0 ?
+V 149 1 0 ?
+I -7 1 0 ?
+D -37 1 0 ?
+V 209 1 0 ?
+C -90 1 0 ?
+K -35 1 0 ?
+L 150 1 0 ?
+T 94 1 1 ?
+I -49 1 0 ?
+A 36 1 1 ?
+V 227 1 1 ?
+V 51 1 0 ?
+R 48 1 1 ?
+G 13 1 0 ?
+M 14 1 1 ?
+E -39 1 0 ?
+D -16 1 1 ?
+R 81 1 1 ?
+K 58 1 0 ?
+K 154 1 0 ?
+C 107 1 0 ?
+R 9 1 1 ?
+Q -34 1 0 ?
+H 49 1 1 ?
+A 185 1 0 ?
+T 195 1 0 ?
+G 70 1 1 ?
+M -33 1 1 ?
+Q 117 1 0 ?
+L 138 1 0 ?
+T -24 1 0 ?
+H 148 1 0 ?
+G 26 1 1 ?
+A 225 1 0 ?
+D 202 1 0 ?
+G 113 1 0 ?
+C 119 1 1 ?
+R -61 1 0 ?
+Y 53 1 1 ?
+D -51 1 0 ?
+S 97 1 1 ?
+L 120 1 0 ?
+I 202 1 0 ?
+T 99 1 1 ?
+H -37 1 1 ?
+N 126 1 1 ?
+K 6 1 0 ?
+L 89 1 1 ?
+L 49 1 0 ?
+G 231 1 0 ?
+S 54 1 0 ?
+K 56 1 1 ?
+E 11 1 0 ?
+W -70 1 1 ?
+H -29 1 0 ?
+F -76 1 1 ?
+M 7 1 0 ?
+V 63 1 0 ?
+A 175 1 0 ?
+K -58 1 0 ?
+F -58 1 0 ?
+V 42 1 1 acetylation
+Q 110 1 0 ?
+F 194 1 0 ?
+N -8 1 0 ?
+V -37 1 1 ?
+C 164 1 0 ?
+K 62 1 1 ?
+F -60 1 1 ?
+R -40 1 0 ?
+C 246 1 0 ?
+D 46 1 1 ?
+G 64 1 0 ?
+P 13 1 0 ?
+L 140 1 0 ?
+L 4 1 0 ?
+R 143 1 0 ?
+W 35 1 1 ?
+F 47 1 0 ?
+R 88 1 0 ?
+C 141 1 0 ?
+D 7 1 1 ?
+H -17 1 0 ?
+Q 35 1 0 ?
+K 49 1 0 ?
+E -40 1 0 ?
+P 49 1 0 ?
+E 155 1 0 ?
+V 24 1 1 ?
+K 82 1 0 ?
+S 37 1 0 ?
+F 101 1 0 ?
+L 201 1 1 ?
+K 160 1 0 ?
+V 184 1 1 ?
+M 42 1 1 acetylation
+Y 68 1 1 ?
+C 97 1 1 ?
+A 126 1 0 ?
+G 189 1 1 ?
+G 61 1 0 ?
+A 23 1 1 ?
+F -72 1 0 ?
+I 222 1 0 ?
+C -94 1 0 ?
+L -40 1 0 ?
+K -44 1 0 ?
+G 126 1 0 ?
+S -9 1 0 ?
+F 120 1 1 ?
+F -5 1 1 ?
+C 81 1 0 ?
+T -34 1 0 ?
+W -117 1 0 ?
+Q -49 1 0 ?
+H 15 1 1 ?
+H 29 1 1 ?
+G 33 1 1 ?
+C -27 1 1 ?
+C 136 1 0 ?
+D 6 1 0 ?
+E 27 1 0 ?
+V -16 1 1 ?
+Q -46 1 0 ?
+A 149 1 0 ?
+F -89 1 0 ?
+E 116 1 1 ?
+L -31 1 0 ?
+T 89 1 0 ?
+H -12 1 0 ?
+R -63 1 0 ?
+A -5 1 0 ?
+H 211 1 1 ?
+H -64 1 1 ?
+S 99 1 0 ?
+E 8 1 0 ?
+L 116 1 0 ?
+L 240 1 0 ?
+T 78 1 1 ?
+S 8 1 0 ?
+H -80 1 0 ?
+I 71 1 1 ?
+K 140 1 0 ?
+I 61 1 1 ?
+G 175 1 0 ?
+W -39 1 0 ?
+M 89 1 0 ?
+P -21 1 0 ?
+E 24 1 0 ?
+L 67 1 0 ?
+T 125 1 0 ?
+C 16 1 0 ?
+G 41 1 0 ?
+M 49 1 1 ?
+P 57 1 0 NT+CAM
+T -7 1 0 ?
+F -44 1 0 ?
+D 44 1 1 ?
+I -15 1 1 ?
+C 219 1 1 ?
+I 125 1 0 ?
+M -71 1 0 ?
+F 206 1 0 ?
+N 144 1 0 ?
+E 66 1 0 ?
+I 187 1 0 ?
+F -12 1 0 ?
+T 130 1 0 ?
+R -94 1 0 ?
+K 223 1 1 ?
+I 57 1 0 NT+CAM
+A 241 1 0 ?
+K -33 1 1 ?
+A 116 1 0 ?
+E -54 1 0 ?
+R -81 1 0 ?
+W 20 1 1 ?
+L 103 1 0 ?
+F 79 1 1 ?
+H -8 1 1 ?
+L 122 1 0 ?
+L 47 1 0 ?
+G 77 1 0 ?
+W -14 1 0 ?
+L 30 1 0 ?
+L 26 1 1 ?
+I 18 1 0 ?
+E -3 1 0 ?
+T 22 1 1 Sodium adduct
+F -62 1 0 ?
+C -14 1 0 ?
+I 35 1 0 ?
+Y -104 1 0 ?
+D 145 1 0 ?
+A 69 1 0 ?
+V -10 1 0 ?
+F -74 1 1 ?
+K 80 1 0 ?
+E 106 1 0 ?
+Y -96 1 0 ?
+L -10 1 0 ?
+L 208 1 0 ?
+R 63 1 0 ?
+F 9 1 0 ?
+L 246 1 0 ?
+H 13 1 1 ?
+A 249 1 0 ?
+M -59 1 0 ?
+Q 19 1 0 ?
+K 237 1 0 ?
+S -30 1 0 ?
+W -80 1 1 ?
+F 196 1 0 ?
+Q -44 1 1 ?
+T 57 1 0 NT+CAM
+E 220 1 0 ?
+P 33 1 1 ?
+I -37 1 0 ?
+Q 5 1 1 ?
+T 84 1 0 ?
+C 76 1 1 ?
+C 159 1 0 ?
+E -64 1 0 ?
+R 109 1 0 ?
+G 157 1 1 ?
+I -23 1 0 ?
+M 53 1 1 ?
+L -54 1 1 ?
+K 119 1 1 ?
+F 69 1 0 ?
+A 167 1 0 ?
+W -108 1 1 ?
+T -36 1 0 ?
+L 193 1 1 ?
+S 107 1 0 ?
+D 228 1 0 ?
+D 112 1 1 ?
+E 33 1 1 ?
+H 48 1 1 ?
+R -38 1 0 ?
+D 237 1 1 ?
+C 134 1 0 ?
+T 64 1 0 ?
+C 229 1 0 ?
+R 117 1 0 ?
+F 153 1 0 ?
+L 31 1 1 ?
+F -63 1 0 ?
+N -51 1 0 ?
+V 7 1 0 ?
+E 108 1 0 ?
+L 169 1 0 ?
+I 36 1 1 ?
+L 27 1 1 ?
+P 15 1 1 ?
+E 90 1 0 ?
+D 61 1 0 ?
+A 85 1 0 ?
+R 85 1 1 ?
+Y -15 1 0 ?
+A 134 1 0 ?
+F -75 1 0 ?
+H -43 1 1 ?
+H 228 1 0 ?
+S 154 1 0 ?
+G 108 1 0 ?
+M -28 1 0 ?
+M 96 1 0 ?
+F -45 1 0 ?
+M -68 1 0 ?
+E 26 1 1 ?
+I 68 1 0 ?
+I 156 1 1 ?
+S -29 1 0 ?
+I 45 1 0 ?
+D 59 1 0 ?
+D -26 1 1 ?
+D 84 1 0 ?
+Y 30 1 1 ?
+K 242 1 0 ?
+K 33 1 0 ?
+N -57 1 0 ?
+L -21 1 0 ?
+P 34 1 0 ?
+E 57 1 1 NT+CAM
+R -75 1 0 ?
+K 203 1 0 ?
+A 34 1 0 ?
+S 122 1 0 ?
+L 82 1 1 ?
+Q 13 1 0 ?
+F 54 1 1 ?
+G 130 1 1 ?
+Q 39 1 1 ?
+S -5 1 0 ?
+L 73 1 0 ?
+H 53 1 1 ?
+G 93 1 0 ?
+T 60 1 0 ?
+D -21 1 0 ?
+L 173 1 0 ?
+D 113 1 1 ?
+E -68 1 1 ?
+Q 235 1 0 ?
+M -15 1 0 ?
+G 110 1 0 ?
+Y 238 1 0 ?
+D -33 1 1 ?
+I -22 1 1 ?
+Q -15 1 0 ?
+S -17 1 1 ?
+V 37 1 0 ?
+Y 27 1 1 ?
+I 6 1 0 ?
+K 54 1 0 ?
+N 94 1 0 ?
+S -23 1 0 ?
+L 65 1 0 ?
+Q 176 1 0 ?
+Q 127 1 0 ?
+N 123 1 0 ?
+H 97 1 0 ?
+V 139 1 0 ?
+A 76 1 0 ?
+K -31 1 1 ?
+V 10 1 0 ?
+Y -88 1 0 ?
+G 68 1 0 ?
+D 125 1 1 ?
+E 18 1 0 ?
+A 66 1 0 ?
+L -44 1 0 ?
+R -25 1 0 ?
+I -43 1 0 ?
+E 115 1 0 ?
+V 127 1 0 ?
+V 220 1 1 ?
+V 85 1 0 ?
+C -62 1 0 ?
+V 165 1 0 ?
+M 33 1 1 ?
+Q -50 1 1 ?
+Q 199 1 0 ?
+T 149 1 1 ?
+V 159 1 1 ?
+E 185 1 0 ?
+D 74 1 1 ?
+S 77 1 1 ?
+F 125 1 0 ?
+T 53 1 1 ?
+D 49 1 0 ?
+K 142 1 1 ?
+N 17 1 1 ?
+I 109 1 0 ?
+V 210 1 0 ?
+H -68 1 0 ?
+L 7 1 0 ?
+F -29 1 0 ?
+E 6 1 1 ?
+L 188 1 0 ?
+T 131 1 0 ?
+N 248 1 0 ?
+S 44 1 1 ?
+R 110 1 0 ?
+L 98 1 0 ?
+G 36 1 0 ?
+Q -36 1 0 ?
+I 116 1 0 ?
+L 62 1 0 ?
+I 242 1 0 ?
+N 40 1 0 ?
+I 120 1 0 ?
+R -95 1 0 ?
+S 15 1 0 ?
+Y 19 1 1 ?
+N 55 1 1 ?
+R 92 1 0 ?
+L 54 1 1 ?
+T 85 1 1 ?
+Y 80 1 0 phosphorylation
+M 198 1 1 ?
+K 138 1 0 ?
+K -9 1 0 ?
+K -15 1 1 ?
+L 241 1 1 ?
+S 191 1 1 ?
+N -30 1 1 ?
+P 53 1 1 ?
+T 12 1 0 ?
+A 83 1 0 ?
+F -18 1 1 ?
+C 105 1 1 ?
+K 129 1 0 ?
+R -87 1 0 ?
+T 98 1 1 ?
+F 65 1 0 ?
+L 5 1 0 ?
+I -11 1 1 ?
+A 32 1 0 ?
+H -14 1 1 ?
+I 131 1 0 ?
+P -24 1 0 ?
+H -9 1 0 ?
+P 38 1 0 Potassium adduct
+P -9 1 0 ?
+T 92 1 1 ?
+K 133 1 0 ?
+N -11 1 1 ?
+Y 48 1 1 ?
+I 212 1 1 ?
+I 182 1 0 ?
+F -65 1 1 ?
+V 94 1 0 ?
+Q -59 1 0 ?
+A 184 1 1 ?
+K -51 1 0 ?
+T 73 1 1 ?
+D 148 1 1 ?
+L -38 1 0 ?
+D 82 1 0 ?
+G 31 1 0 ?
+S 95 1 1 ?
+K 211 1 0 ?
+M 215 1 0 ?
+M 77 1 0 ?
+F -17 1 1 ?
+A 170 1 0 ?
+E 62 1 1 ?
+H 129 1 0 ?
+G 243 1 0 ?
+E -33 1 1 ?
+E -34 1 0 ?
+G 137 1 0 ?
+K 163 1 0 ?
+H 62 1 0 ?
+T -14 1 0 ?
+D 8 1 1 ?
+V -29 1 0 ?
+Y 17 1 1 ?
+L 210 1 1 ?
+T 153 1 0 ?
+D 23 1 0 ?
+Y -6 1 1 ?
+A 111 1 0 ?
+K 145 1 0 ?
+L 156 1 0 ?
+Y 150 1 0 ?
+W -92 1 0 ?
+Y -55 1 1 ?
+A 127 1 0 ?
+K 40 1 1 ?
+K -21 1 0 ?
+D 116 1 0 ?
+G 219 1 0 ?
+K 35 1 1 ?
+D 100 1 0 ?
+A 222 1 1 ?
+T -13 1 1 ?
+I 233 1 0 ?
+N 175 1 0 ?
+G 122 1 0 ?
+Q 223 1 0 ?
+R 107 1 0 ?
+C -65 1 0 ?
+Y -57 1 0 ?
+E -72 1 1 ?
+V -2 1 0 ?
+G 74 1 0 ?
+I -27 1 0 ?
+C -93 1 0 ?
+L 34 1 0 ?
+H 117 1 0 ?
+V 47 1 0 ?
+L 160 1 0 ?
+L 42 1 1 acetylation
+L -46 1 1 ?
+R 25 1 0 ?
+S 96 1 0 ?
+V -39 1 0 ?
+A 173 1 0 ?
+K 233 1 0 ?
+W 13 1 1 ?
+E 240 1 0 ?
+W 44 1 1 ?
+F -40 1 1 ?
+C -25 1 1 ?
+C 29 1 0 ?
+T 108 1 0 ?
+P 249 1 0 ?
+R 128 1 1 lysine adduct
+Y 6 1 0 ?
+N 129 1 1 ?
+R -42 1 0 ?
+A 200 1 0 ?
+A 26 1 0 ?
+G 92 1 0 ?
+T -26 1 0 ?
+R -56 1 0 ?
+S 83 1 0 ?
+F 248 1 0 ?
+I 79 1 1 ?
+A -9 1 0 ?
+I 74 1 1 ?
+V -9 1 0 ?
+I 112 1 1 ?
+R 141 1 0 ?
+I 72 1 1 ?
+N 41 1 0 ?
+H 72 1 1 ?
+Q 122 1 0 ?
+R 55 1 0 R+55
+G 180 1 1 ?
+G 102 1 1 ?
+P 213 1 0 ?
+A 192 1 0 ?
+F 178 1 0 ?
+V 114 1 0 ?
+D -58 1 1 ?
+T 154 1 1 ?
+R 44 1 0 ?
+D 92 1 0 ?
+K 53 1 1 ?
+K -34 1 0 ?
+F 127 1 1 ?
+I 141 1 0 ?
+E 122 1 0 ?
+Q 9 1 1 ?
+Q -3 1 1 ?
+Q -57 1 0 ?
+Q 68 1 0 ?
+Q 36 1 1 ?
+Y -103 1 0 ?
+S 74 1 0 ?
+G 124 1 1 ?
+W 57 1 1 NT+CAM
+Y -10 1 1 ?
+D 47 1 0 ?
+M 8 1 0 ?
+V 116 1 1 ?
+H 61 1 0 ?
+I 58 1 0 ?
+Y 13 1 1 ?
+N 43 1 1 carbamylation
+K 187 1 0 ?
+K -39 1 0 ?
+K 174 1 0 ?