Automated Proteogenomic Analysis of Shewanella Putrefaciens CN-32.



Peptide Identification

Number of genomic elements = 1
Name Length
Chromsome14649325

Number of Spectra: 768367

Peptide Spectra
IDs on Actual Database 26626 157776
IDs on Decoy Database 1205 1697
False Discovery Rate 0.045 0.011

The above mentioned false discovery rates are computed in the database search using a six-frame translation of the genome and a decoy database of the same size. The false discovery rates, counting only the hits in the coding regions and using decoy database of the size of proteome (more common practise), are lower: 0.002 at the spectrum level and 0.007 at the peptide level.

Access List of Identified Peptides


Protein Identification

Number of annotated ORFs = 3972
Number of proteins confirmed with 2 or more peptides = 1745 | Access List
Number of proteins having only 1 peptide = 534 | Access List


The histogram of number of unique peptides per protein. X axis has the number of unique peptides per protein, Y axis has the frequency (number of proteins). Note that the bins are of size 2, and the plot is truncated at 100 (there may be proteins that have more peptides).



The histogram of percent coverage of the proteins by the identified peptides. X axis has the coverage in percent, Y axis has the frequency (number of proteins). The bins are of size 10 percentage points.




Improving Genome Annotation

Number of peptides within genes: 24949
Number of peptides crossing gene boundaries: 32

Newly Identified Protein Coding Segments:

CoordinatesLength#Peptides#SpectraPossibleExplanation
Seq1:381472-381516 (+1)1525Unknown
Seq1:590101-590286 (+1)6212N_Term_Extension of CN32_0580
Seq1:1687150-1687395 (+1)8225Unknown
Seq1:1999015-1999074 (+1)2011Unknown
Seq1:2108431-2108535 (+1)3511N_Term_Extension of CN32_1882
Seq1:2583910-2584170 (+1)8722New Gene
Seq1:2881000-2881005 (+1)212N_Term_Extension of CN32_2512
Seq1:4196875-4196901 (+1)923Unknown
Seq1:4581937-4581945 (+1)311N_Term_Extension of CN32_3984
Seq1:4623169-4623396 (+1)7624New Gene
Seq1:812651-812860 (+2)7011N_Term_Extension of CN32_0769
Seq1:1534586-1534666 (+2)2711N_Term_Extension of CN32_1389
Seq1:1570487-1570504 (+2)611N_Term_Extension of CN32_1418
Seq1:1599272-1599385 (+2)3811N_Term_Extension of CN32_1445
Seq1:1672637-1672825 (+2)6312N_Term_Extension of CN32_1502
Seq1:1687916-1688182 (+2)8946New Gene
Seq1:1776749-1776904 (+2)5211N_Term_Extension of CN32_1597
Seq1:1885361-1885489 (+2)4322New Gene
Seq1:2001155-2001262 (+2)3611Unknown
Seq1:2406617-2406691 (+2)2523New Gene
Seq1:2843129-2843146 (+2)611N_Term_Extension of CN32_2487
Seq1:3304055-3304150 (+2)3233New Gene
Seq1:3604760-3604855 (+2)3211Unknown
Seq1:3892799-3892825 (+2)922Unknown
Seq1:4642622-4642723 (+2)3411N_Term_Extension of CN32_0027
Seq1:927027-927035 (+3)311N_Term_Extension of CN32_0874
Seq1:1658076-1659086 (+3)33729248Unknown
Seq1:1746405-1746407 (+3)111N_Term_Extension of CN32_1572
Seq1:2220540-2220653 (+3)3822Unknown
Seq1:3542253-3542354 (+3)3423Unknown
Seq1:3734463-3734591 (+3)4311N_Term_Extension of CN32_3250
Seq1:3810585-3810614 (+3)1011Unknown
Seq1:4126899-4127270 (+3)12459Unknown
Seq1:4553440-4553529 (-1)3011N_Term_Extension of CN32_3954
Seq1:4531951-4532055 (-1)3511Unknown
Seq1:3575812-3575841 (-1)1013N_Term_Extension of CN32_3110
Seq1:3234187-3234195 (-1)313N_Term_Extension of CN32_2820
Seq1:3140326-3140385 (-1)2013N_Term_Extension of CN32_2747
Seq1:2911630-2911728 (-1)3322N_Term_Extension of CN32_2536
Seq1:2518078-2518113 (-1)1211N_Term_Extension of CN32_2222
Seq1:2199640-2199789 (-1)5011Unknown
Seq1:1218343-1218534 (-1)6411N_Term_Extension of CN32_1120
Seq1:1003444-1003668 (-1)7522Unknown
Seq1:449695-449877 (-1)61270Unknown
Seq1:4264995-4265009 (-2)514N_Term_Extension of CN32_3706
Seq1:4163619-4163666 (-2)1611N_Term_Extension of CN32_3614
Seq1:4021125-4021154 (-2)1013Unknown
Seq1:2994150-2994170 (-2)711N_Term_Extension of CN32_2615
Seq1:2823072-2823149 (-2)2622New Gene
Seq1:2265510-2265734 (-2)7522New Gene
Seq1:2258202-2258315 (-2)38850N_Term_Extension of CN32_2017
Seq1:2192643-2192654 (-2)411N_Term_Extension of CN32_1949
Seq1:1814085-1814204 (-2)4011N_Term_Extension of CN32_1629
Seq1:1780230-1780592 (-2)121416New Gene
Seq1:1558668-1558760 (-2)3111N_Term_Extension of CN32_1410
Seq1:1150242-1150283 (-2)1423New Gene
Seq1:1093692-1093694 (-2)111N_Term_Extension of CN32_0993
Seq1:761610-761747 (-2)4611N_Term_Extension of CN32_0714
Seq1:50148-50279 (-2)4422Unknown
Seq1:4326842-4326844 (-3)111N_Term_Extension of CN32_3768
Seq1:2929307-2929465 (-3)5311Unknown
Seq1:1878197-1878223 (-3)922Unknown
Seq1:1160519-1160545 (-3)911N_Term_Extension of CN32_0932
Seq1:1148861-1148869 (-3)311N_Term_Extension of CN32_0940
Seq1:366845-366997 (-3)5113N_Term_Extension of CN32_0377

Each segment represents a putative coding region that is not contained within known ORFs. The number of peptides reported in this table includes only the peptides that lie outside the known ORFs. If the number is 1, it means that there must be another peptide nearby within a known ORF, since we require atleast two peptides for any new segment.



Analyzing Non-tryptic Peptides

Number of peptides with both end non-tryptic = 764
Number of peptides with one end non-tryptic = 7565

Number of contained peptides = 5832
- Peptides that are contained at their N-terminal side = 2533
- Peptides that are contained at their C-terminal side = 3496

Number of non-Contained peptides (i.e. peptides that are not a subsequence of other peptides) = 2497
Number of non-Covered peptides (non-Contained and located within confirmed proteins) = 1362
Number of non-Covered Ntermini (note that one terminus of a nonCovered peptide may be covered; here we look for cases where N-terminus is not covered) = 538
Number of non-Covered Ntermini with no upstream coverage in the protein = 327

The plot gives the distribution of residues distances from a non-tryptic end-point to the endpoint in the containing tryptic peptide. X axis shows the distance and Y axis shows the frequency.



Distribution of the N-termini of all non-covered peptides, and of those which also have no upstream coverage. X axis shows the distance of the peptide from N-terminus of the protein, and Y shows has the frequency.




N-Terminal Methionine Cleavage


195 proteins found with N-terminal Methionine Cleavages


Efficiency factor of N-terminal methionine cleavage for each amino acid at second position, as observed in this proteomic analysis and in-vitro analysis of E. coli [Hirel et al. 1989]. The amino acids are ordered in the increasing order of the size of their side chains. Amino acids that occur less than 10 times at the second position are shown with an asterisk.



AminoAcid FrequencyCut FrequencyNoCut CleavageEfficiency HirelEtAlEfficiency
G 5 2 0.714285714285714 0.971
A 50 0 1 0.958
P 16 0 1 0.882
S 84 7 0.923076923076923 0.84
T 37 19 0.660714285714286 0.897
V 1 2 0.333333333333333 0.837
C 0 0 NA 0.71
N 0 28 0 0.164
D 0 11 0 0.161
L 0 13 0 0.163
I 1 20 0.0476190476190476 0.184
H 0 7 0 0
Q 0 19 0 0
E 0 9 0 0
F 0 6 0 0
M 1 0 1 0
K 0 43 0 0
Y 0 4 0 0
W 0 1 0 0
R 0 4 0 0

The table reports the N-terminal methionine cleavage efficiency for different amino acids at second position. For each amion acid at second position, the number of proteins with a N-terminal methionine cleavage and with no cleavage are reported, and compared with the efficiency values reported in [Hirel et al. 1989].




Signal Peptides


Venn diagram of all signal peptide predictions on confirmed proteins.



Sequence logo for the amino acid sequences motif of all signal peptides identified by our analysis. Position -1 corresponds to the last residue of the signal peptide.



Number of signal predictions by SignalP and Predisi rejected by us based on observation of peptides upstream of the signal cleavage site.



Unrestricted Modifications Search

Identified 6995 unique modification sites in the organism, at 5% false discovery rate. These are observed by 7470 peptides in the forward database (377 in the shuffled database). Note that two or more different peptide species may correspond to the same modification site.


AminoAcidModificationMassNumPeptidesPeptidesWithUnmodifiedVersionsPossibleAnnotation
+M 16 1835 1607 oxidation M
+W 32 282 251 double oxidation W
+Q -17 226 167 pyroglutamate formation
+S 28 206 167 formaldehyde S
+W 4 166 149 Oxidation (Trp to kynurenine)
+D 22 165 142 Sodium adduct
+N -17 164 137 succinimide formation
+T 28 145 125 formaldehyde T
+K 28 123 114 dimethylation
+E 22 105 85 Sodium adduct
+K 43 82 56 carbamylation K
+M 32 78 62 double oxidation M
+D -18 76 53 dehydration
+V 22 76 63 Sodium adduct
+T -18 76 47 dehydration
+H 28 71 70 ?
+C -57 56 8 C-57
+G 22 48 44 Sodium adduct
+N 22 45 40 Sodium adduct
+Y 14 42 42 ?
+L -17 40 34 ?
+V 28 38 36 ?
+D 53 32 31 ?
+C 62 30 6 ?
+L 22 29 19 Sodium adduct
+W 16 28 25 oxidation W
+L 28 26 23 ?
+M -48 24 12 M+16-64
+A 22 23 18 Sodium adduct
+E -18 21 11 dehydration
+I 28 21 21 ?
+I -17 21 19 ?
+Y 16 21 21 ?
+E -17 19 15 ?
+K 14 19 10 methylation
+D -17 18 16 ?
+C -9 18 1 ?
+E 28 18 18 ?
+T 22 17 14 Sodium adduct
+D 28 17 17 ?
+A 28 17 17 ?
+C 19 17 3 ?
+W 48 17 17 ?
+M 19 16 15 ?
+L -18 16 10 ?
+N 3 15 15 ?
+S -18 15 8 dehydration
+Q 28 14 14 ?
+N 28 14 14 ?
+E 53 13 12 ?
+L 38 13 6 Potassium adduct
+V -17 13 13 ?
+C 77 13 4 ?
+R 28 13 13 ?
+L 53 13 12 ?
+C 93 13 5 ?
+G 28 12 12 ?
+F 28 11 11 ?
+A 38 10 7 Potassium adduct
+L 14 10 8 ?
+V -18 10 6 ?
+V 53 9 8 ?
+A 16 9 6 ?
+C -11 9 1 ?
+M 3 9 9 ?
+M 48 9 7 ?
+K 128 9 8 lysine adduct
+L 16 8 4 ?
+K 38 8 4 Potassium adduct
+Y 15 8 8 ?
+Y -97 8 1 ?
+K -18 8 7 ?
+E -14 7 7 ?
+Q 14 7 2 ?
+S 48 7 5 ?
+A 53 7 7 ?
+C 12 7 0 ?
+I -14 7 7 ?
+M 38 7 4 Potassium adduct
+N 53 7 7 ?
+C 109 7 4 ?
+D 128 7 4 lysine adduct
+M 17 7 7 ?
+R 16 6 1 ?
+Y 53 6 6 ?
+A 43 6 3 carbamylation
+T 53 6 6 ?
+P 3 6 6 ?
+L -9 6 0 ?
+A 40 6 0 ?
+A 50 6 3 ?
+P 48 6 6 ?
+T -17 6 5 ?
+F 16 6 5 ?
+E 38 6 1 Potassium adduct
+K 100 6 5 ?
+C 7 6 0 ?
+H -17 5 4 ?
+G 53 5 5 ?
+I 53 5 4 ?
+C 219 5 2 ?
+D 54 5 5 ?
+Q 3 5 5 ?
+C 63 5 2 ?
+F -17 5 4 ?
+V -12 5 2 ?
+A 48 5 2 ?
+V 3 5 5 ?
+C 218 5 4 ?
+C -84 5 0 ?
+G 44 5 1 ?
+A 14 5 4 ?
+Q 22 5 4 Sodium adduct
+V 18 5 1 ?
+A 44 5 4 ?
+D 14 5 3 ?
+P 16 5 4 ?
+S 14 5 5 ?
+M -16 5 3 ?
+D 32 5 2 ?
+V 57 5 2 NT+CAM
+S 22 5 4 Sodium adduct
+L 3 4 3 ?
+W 12 4 3 Formaldehyde adduct
+N 44 4 3 ?
+V 14 4 3 ?
+H 29 4 4 ?
+L 12 4 2 ?
+E 27 4 2 ?
+L 67 4 1 ?
+C -83 4 0 ?
+Y 28 4 4 ?
+C 229 4 2 ?
+L 128 4 1 lysine adduct
+S 53 4 4 ?
+L 65 4 2 ?
+K 19 4 3 ?
+S 80 4 0 phosphorylation
+E -56 4 1 ?
+E 3 4 3 ?
+L 54 4 1 ?
+P 53 4 4 ?
+I 15 4 3 ?
+C 40 4 0 ?
+M -32 4 2 ?
+W 44 4 4 ?
+V -32 4 3 ?
+F 17 4 3 ?
+C 200 4 0 ?
+T 128 4 0 lysine adduct
+I 12 4 1 ?
+E 47 4 1 ?
+W 15 4 4 ?
+N -56 4 0 ?
+A 19 4 2 ?
+F 57 4 2 NT+CAM
+D 19 4 4 ?
+E 54 4 2 ?
+I 54 4 4 ?
+G 42 4 2 acetylation
+C -32 4 1 ?
+L 35 4 1 ?
+D 24 4 4 ?
+M 44 4 4 ?
+L 57 4 0 NT+CAM
+S 57 4 4 NT+CAM
+E -27 4 0 ?
+Q -61 4 1 ?
+C 232 4 2 ?
+Q 53 4 3 ?
+C 190 4 1 ?
+I 43 4 1 carbamylation
+D -13 4 3 ?
+V -14 4 2 ?
+S 156 4 3 ?
+C 33 3 0 ?
+R -70 3 0 ?
+L 88 3 0 ?
+K 57 3 0 NT+CAM
+V 42 3 2 acetylation
+K 131 3 1 ?
+E -30 3 2 ?
+T 100 3 2 ?
+V 16 3 2 ?
+L 77 3 1 ?
+L -33 3 1 ?
+S 102 3 0 phosphorylation + sodium
+D 87 3 0 ?
+A 74 3 2 ?
+M 49 3 2 ?
+L -5 3 0 ?
+G 3 3 2 ?
+C 248 3 1 ?
+S 41 3 3 ?
+C 159 3 2 ?
+L -54 3 1 ?
+L -42 3 0 ?
+L 18 3 2 ?
+L 40 3 0 ?
+L 31 3 0 ?
+A 99 3 0 ?
+L 19 3 1 ?
+I -18 3 1 ?
+H 53 3 3 ?
+I -22 3 1 ?
+T 16 3 1 ?
+F 87 3 1 ?
+T 12 3 1 ?
+Y 48 3 2 ?
+L -56 3 0 ?
+A 31 3 2 ?
+K -51 3 0 ?
+T 48 3 2 ?
+V 15 3 2 ?
+T -14 3 3 ?
+Y 17 3 3 ?
+L 64 3 1 ?
+A 10 3 1 ?
+L 34 3 2 ?
+L -46 3 1 ?
+M -7 3 0 ?
+N -18 3 2 ?
+Q -18 3 1 ?
+A 26 3 0 ?
+C -100 3 0 ?
+E -47 3 1 ?
+E -60 3 1 ?
+R 44 3 2 ?
+V 43 3 1 carbamylation
+A 133 3 1 ?
+S 5 3 3 ?
+W 60 3 2 ?
+E -31 3 2 ?
+N 100 3 0 ?
+A 4 3 3 ?
+K 81 3 2 ?
+A -13 3 0 ?
+E 136 3 2 ?
+V 31 3 2 ?
+D 3 3 3 ?
+L 48 3 1 ?
+F 31 3 2 ?
+A 75 3 1 ?
+A 204 3 2 ?
+A 115 3 0 ?
+L -43 3 0 ?
+D 48 3 2 ?
+E -35 3 1 ?
+H -69 3 2 ?
+L 43 3 2 carbamylation
+L 134 3 0 ?
+D -52 3 0 ?
+E 15 3 2 ?
+L -55 3 0 ?
+L -30 3 1 ?
+S -14 3 2 ?
+K 156 3 3 ?
+S -21 3 0 ?
+E -71 3 1 ?
+K 30 3 2 ?
+I 3 3 3 ?
+G 84 3 2 ?
+A 33 3 0 ?
+S -24 3 0 ?
+G 94 3 1 ?
+I -30 3 1 ?
+D 43 3 2 carbamylation
+L 33 3 0 ?
+W 7 3 3 ?
+S -16 3 3 ?
+E 4 3 3 ?
+Y 3 3 3 ?
+E 85 3 1 ?
+D 16 3 3 ?
+E 25 3 2 ?
+C -99 3 0 ?
+Y 33 3 1 ?
+V -13 3 1 ?
+D 155 2 1 ?
+D 111 2 2 ?
+E 73 2 0 ?
+G 97 2 0 ?
+T 5 2 2 ?
+A 96 2 1 ?
+D 121 2 2 ?
+M 28 2 2 ?
+A 3 2 2 ?
+K -12 2 1 ?
+I -7 2 1 ?
+C -10 2 1 ?
+F 3 2 2 ?
+M 59 2 1 ?
+E 29 2 1 ?
+T -24 2 0 ?
+L 212 2 0 ?
+V 71 2 1 ?
+G 90 2 0 ?
+D -51 2 0 ?
+E -36 2 0 ?
+L -25 2 1 ?
+M 35 2 1 ?
+H -29 2 1 ?
+F -76 2 0 ?
+N -8 2 0 ?
+L -47 2 0 ?
+I 14 2 2 ?
+E 82 2 0 ?
+V 68 2 0 ?
+V 214 2 1 ?
+R -91 2 0 ?
+D 7 2 1 ?
+E -40 2 1 ?
+K 82 2 1 ?
+L 72 2 2 ?
+A 104 2 0 ?
+F -34 2 1 ?
+E 127 2 1 ?
+G 160 2 0 ?
+I -25 2 1 ?
+H 100 2 0 ?
+L -40 2 1 ?
+Y -17 2 2 ?
+Q 87 2 1 ?
+E -21 2 0 ?
+L -52 2 0 ?
+D 69 2 1 ?
+L -31 2 0 ?
+L 116 2 1 ?
+T 166 2 0 ?
+S 121 2 0 ?
+D -48 2 0 ?
+W 14 2 2 ?
+I 206 2 1 ?
+D 44 2 0 ?
+D -5 2 0 ?
+G 35 2 1 ?
+E 66 2 0 ?
+A 227 2 1 ?
+R -94 2 0 ?
+L 47 2 1 ?
+C 162 2 1 ?
+I 32 2 2 ?
+G 214 2 0 ?
+L 26 2 0 ?
+N 141 2 0 ?
+L -10 2 0 ?
+S -30 2 2 ?
+L -49 2 0 ?
+Q 27 2 2 ?
+G 157 2 0 ?
+D -27 2 1 ?
+A -14 2 1 ?
+R 71 2 1 ?
+E 49 2 0 ?
+A 35 2 0 ?
+D 175 2 0 ?
+T -3 2 0 ?
+V 7 2 2 ?
+G 38 2 1 Potassium adduct
+P 15 2 2 ?
+L 220 2 0 ?
+R 5 2 0 ?
+D -32 2 0 ?
+F -75 2 1 ?
+T 67 2 0 ?
+A 81 2 1 ?
+A 194 2 0 ?
+I 156 2 0 ?
+F 140 2 0 ?
+I -36 2 0 ?
+L 82 2 1 ?
+F 54 2 2 ?
+V 49 2 1 ?
+F 15 2 2 ?
+W -97 2 0 ?
+E -26 2 2 ?
+N 94 2 2 ?
+T -29 2 1 ?
+E 43 2 1 carbamylation
+L 102 2 0 ?
+G 59 2 0 ?
+C 94 2 0 ?
+L 36 2 0 ?
+P 43 2 0 carbamylation
+A 24 2 1 ?
+V 85 2 0 ?
+A 64 2 0 ?
+P 64 2 1 ?
+W 49 2 2 ?
+W 30 2 2 ?
+L 188 2 0 ?
+L 62 2 0 ?
+V 32 2 1 ?
+I 51 2 1 ?
+A 58 2 1 ?
+E 77 2 0 ?
+Y 80 2 0 phosphorylation
+V 90 2 0 ?
+F 32 2 2 ?
+S 142 2 0 ?
+S 196 2 1 ?
+V 5 2 1 ?
+P 63 2 1 ?
+Y 32 2 2 ?
+Y -98 2 0 ?
+A -12 2 1 ?
+A 91 2 1 ?
+Q 11 2 0 ?
+G 8 2 0 ?
+I -33 2 1 ?
+K 40 2 0 ?
+D 100 2 1 ?
+T 232 2 1 ?
+D 15 2 1 ?
+C -72 2 0 ?
+A 62 2 0 ?
+W -115 2 0 ?
+L 42 2 0 acetylation
+S 67 2 0 ?
+N 83 2 0 ?
+W 13 2 2 ?
+C 225 2 2 ?
+R -27 2 2 ?
+E 13 2 0 ?
+Q 72 2 1 ?
+V 54 2 2 ?
+I 145 2 0 ?
+T 154 2 0 ?
+R -55 2 0 ?
+Y 71 2 1 ?
+K 53 2 2 ?
+D 5 2 2 ?
+Q -3 2 0 ?
+H 60 2 2 ?
+A 105 2 0 ?
+S 58 2 0 ?
+Y -95 2 0 ?
+A 20 2 0 ?
+Y 13 2 2 ?
+G 200 2 0 ?
+N 29 2 2 ?
+G 49 2 0 ?
+I 13 2 2 ?
+R -97 2 0 ?
+A 30 2 1 ?
+S -28 2 1 ?
+A 52 2 1 ?
+H -11 2 2 ?
+T 54 2 1 ?
+L -37 2 0 ?
+I 56 2 2 ?
+G 215 2 0 ?
+R -60 2 1 ?
+D 35 2 1 ?
+G 184 2 1 ?
+D 115 2 0 ?
+V 228 2 0 ?
+I 171 2 0 ?
+I -24 2 1 ?
+V -11 2 0 ?
+A 86 2 1 ?
+G 12 2 0 ?
+I 31 2 1 ?
+K -32 2 1 ?
+F -13 2 0 ?
+D -50 2 1 ?
+L 126 2 0 ?
+Q 29 2 1 ?
+L 144 2 0 ?
+G 54 2 1 ?
+L 70 2 1 ?
+N -49 2 1 ?
+L 13 2 1 ?
+P -18 2 1 ?
+N 51 2 0 ?
+S 152 2 1 ?
+L 17 2 1 ?
+T 56 2 1 ?
+A 17 2 1 ?
+Q -47 2 0 ?
+V 44 2 1 ?
+E 139 2 1 ?
+M 64 2 1 ?
+G 63 2 0 ?
+N -7 2 1 ?
+L -53 2 0 ?
+N 10 2 0 ?
+G 34 2 0 ?
+E -9 2 1 ?
+A 59 2 0 ?
+T 8 2 0 ?
+C -43 2 1 methylation
+D 29 2 1 ?
+L 87 2 1 ?
+L 25 2 0 ?
+Q -69 2 1 ?
+M -67 2 0 ?
+E 50 2 0 ?
+V 50 2 1 ?
+E 243 2 1 ?
+E 19 2 2 ?
+Q -52 2 0 ?
+V 36 2 0 ?
+E -32 2 0 ?
+Q -27 2 1 ?
+V 86 2 1 ?
+L 6 2 2 ?
+Y -105 2 0 ?
+H 19 2 2 ?
+C -101 2 0 ?
+I 115 2 1 ?
+Q 226 2 0 ?
+Q 70 2 0 ?
+S 112 2 1 ?
+Y -14 2 1 ?
+V -38 2 0 ?
+E 32 2 0 ?
+G 51 2 1 ?
+C 78 2 0 ?
+E 174 2 1 ?
+I 62 2 0 ?
+I 22 2 2 Sodium adduct
+R -89 2 0 ?
+N -12 2 1 ?
+F 18 2 1 ?
+V 38 2 2 Potassium adduct
+K 9 2 1 ?
+K -61 2 0 ?
+Q -30 2 0 ?
+L 200 2 1 ?
+Y -94 2 0 ?
+C 34 2 1 ?
+I 199 2 1 ?
+V 48 2 1 ?
+Y 29 2 1 ?
+S 229 2 0 ?
+E -50 2 2 ?
+Y -51 2 0 ?
+T 17 2 1 ?
+F -88 2 1 ?
+Q -26 2 1 ?
+E -67 2 1 ?
+V 97 2 0 ?
+E -57 2 1 ?
+A 29 2 1 ?
+L -14 2 2 ?
+N -23 2 1 ?
+T 80 2 0 phosphorylation
+E -61 2 1 ?
+G 227 2 1 ?
+E -46 2 2 ?
+S 59 2 1 ?
+L 119 2 0 ?
+Q 17 2 1 ?
+D -40 2 0 ?
+F -21 2 1 ?
+D 65 2 0 ?
+A 49 2 1 ?
+R 15 2 0 ?
+A 108 2 1 ?
+Y -80 2 0 ?
+T 25 2 1 ?
+A -10 2 0 ?
+I -13 1 1 ?
+U -11 1 0 ?
+D -8 1 1 ?
+A 89 1 0 ?
+T 209 1 0 ?
+M -73 1 1 ?
+V 58 1 0 ?
+Q 16 1 1 ?
+S -26 1 0 ?
+E 170 1 0 ?
+R -9 1 0 ?
+E -5 1 0 ?
+F 135 1 0 ?
+Y 5 1 0 ?
+T 173 1 0 ?
+L 163 1 0 ?
+F -49 1 0 ?
+N 23 1 1 ?
+F -15 1 1 ?
+R 31 1 0 ?
+L -16 1 0 ?
+S 26 1 1 ?
+E 61 1 0 ?
+F -26 1 0 ?
+I -47 1 1 ?
+K 107 1 0 ?
+I 91 1 0 ?
+T 132 1 0 ?
+S 60 1 1 ?
+R -32 1 0 ?
+F -56 1 0 ?
+N -28 1 0 ?
+M 70 1 1 ?
+H -30 1 1 ?
+P -26 1 0 ?
+Q 7 1 0 ?
+V -4 1 1 ?
+F 50 1 1 ?
+I 84 1 0 ?
+R 48 1 1 ?
+I 166 1 0 ?
+S -10 1 0 ?
+D -16 1 1 ?
+Q 48 1 1 ?
+R -83 1 0 ?
+K 154 1 0 ?
+E -52 1 0 ?
+R 9 1 0 ?
+Q 20 1 0 ?
+F 70 1 1 ?
+R 93 1 0 ?
+P 98 1 0 ?
+H 27 1 0 ?
+Y 99 1 0 ?
+I 101 1 0 ?
+D 71 1 0 ?
+P 40 1 0 ?
+M -33 1 1 ?
+R -67 1 0 ?
+L -7 1 0 ?
+H 79 1 0 ?
+M -69 1 1 ?
+P 201 1 0 ?
+T 248 1 1 ?
+H 94 1 0 ?
+A 18 1 1 ?
+L 100 1 0 ?
+T 44 1 1 ?
+C 119 1 1 ?
+T 19 1 0 ?
+D -56 1 0 ?
+E 46 1 0 ?
+H 76 1 0 ?
+L 105 1 0 ?
+Y -102 1 1 ?
+G 231 1 0 ?
+F -82 1 0 ?
+S 54 1 1 ?
+A 130 1 0 ?
+L 84 1 0 ?
+L 226 1 0 ?
+Q 25 1 0 ?
+L 247 1 0 ?
+V 63 1 1 ?
+S 105 1 0 ?
+V -37 1 0 ?
+P 19 1 1 ?
+P 22 1 1 Sodium adduct
+V 115 1 0 ?
+Q -16 1 0 ?
+G 64 1 0 ?
+P -31 1 0 ?
+D 46 1 0 ?
+V 45 1 0 ?
+L 4 1 1 ?
+Q -55 1 0 ?
+R 143 1 0 ?
+W -67 1 0 ?
+I 127 1 0 ?
+N 78 1 1 ?
+C 141 1 0 ?
+T 161 1 0 ?
+W 90 1 0 ?
+V 180 1 0 ?
+K 49 1 1 ?
+K 44 1 0 ?
+V 89 1 0 ?
+E 111 1 0 ?
+K 15 1 0 ?
+I 37 1 0 ?
+K -68 1 0 ?
+Y -81 1 0 ?
+D 193 1 0 ?
+Y -26 1 1 ?
+L 201 1 0 ?
+F -23 1 0 ?
+P 26 1 0 ?
+F 155 1 0 ?
+V 174 1 1 ?
+G 189 1 0 ?
+I 168 1 0 ?
+L 213 1 0 ?
+G 61 1 0 ?
+S 184 1 0 ?
+V -20 1 0 ?
+E 206 1 1 ?
+F -32 1 0 ?
+L 39 1 0 ?
+T 233 1 0 ?
+D -29 1 0 ?
+E 138 1 0 ?
+V 77 1 0 ?
+S -9 1 0 ?
+T -34 1 0 ?
+R 73 1 0 ?
+C -12 1 0 ?
+R 57 1 0 NT+CAM
+A 199 1 0 ?
+I -38 1 0 ?
+S 78 1 0 ?
+N 179 1 0 ?
+G 33 1 1 ?
+C -27 1 0 ?
+D 173 1 1 ?
+W 56 1 1 ?
+D 6 1 0 ?
+V -16 1 0 ?
+S 207 1 0 ?
+E 202 1 0 ?
+H -12 1 1 ?
+G 43 1 0 carbamylation
+A -5 1 0 ?
+E 8 1 1 ?
+F 13 1 1 ?
+G 132 1 0 ?
+E -13 1 1 ?
+R -50 1 0 ?
+L 170 1 0 ?
+E 63 1 0 ?
+D -31 1 1 ?
+H -80 1 1 ?
+T 55 1 1 ?
+Q 30 1 1 ?
+D 204 1 0 ?
+L -22 1 0 ?
+E 24 1 1 ?
+L -23 1 0 ?
+N -6 1 1 ?
+P 20 1 1 ?
+H 180 1 0 ?
+G 149 1 0 ?
+V 23 1 0 ?
+T -7 1 0 ?
+W -119 1 0 ?
+V 119 1 0 ?
+C 220 1 1 ?
+Q -21 1 0 ?
+G 15 1 0 ?
+E 17 1 1 ?
+W -55 1 0 ?
+F -69 1 0 ?
+L 157 1 0 ?
+E -70 1 0 ?
+T 72 1 0 ?
+F 5 1 0 ?
+D 90 1 1 ?
+A 132 1 0 ?
+M -71 1 0 ?
+D 156 1 1 ?
+S -12 1 1 ?
+Q 90 1 0 ?
+A 144 1 0 ?
+E 51 1 0 ?
+A 120 1 0 ?
+K 223 1 0 ?
+L 86 1 0 ?
+K 69 1 0 ?
+Y 113 1 1 ?
+G 206 1 0 ?
+F -47 1 1 ?
+Q 88 1 1 ?
+C 125 1 1 ?
+V 140 1 0 ?
+W 20 1 1 ?
+L 103 1 0 ?
+M -23 1 0 ?
+K 66 1 1 ?
+Q 55 1 0 ?
+E 187 1 1 ?
+C -31 1 0 ?
+P 117 1 1 ?
+L 122 1 0 ?
+K 234 1 0 ?
+N 48 1 1 ?
+K 167 1 0 ?
+C 5 1 0 ?
+V 91 1 0 ?
+V 11 1 0 ?
+G 165 1 0 ?
+L 97 1 0 ?
+Q -39 1 0 ?
+I 179 1 0 ?
+Y -36 1 0 ?
+F -62 1 0 ?
+C -14 1 1 ?
+Y -86 1 0 ?
+Y 66 1 0 ?
+H 216 1 0 ?
+R 70 1 0 ?
+C 175 1 1 ?
+K 80 1 0 ?
+P -19 1 1 ?
+L 208 1 1 ?
+C -96 1 0 ?
+M 227 1 1 ?
+A 214 1 1 ?
+M -45 1 1 ?
+C 230 1 0 ?
+Y -59 1 0 ?
+M 163 1 0 ?
+F 112 1 1 ?
+K -53 1 0 ?
+Q 19 1 1 ?
+K 237 1 1 ?
+I 86 1 0 ?
+D 13 1 0 ?
+V 231 1 0 ?
+V 225 1 0 ?
+C 44 1 0 ?
+F 196 1 0 ?
+T 157 1 0 ?
+R 156 1 0 ?
+A 102 1 0 ?
+I -37 1 1 ?
+P 33 1 1 ?
+T 104 1 0 ?
+C 76 1 1 ?
+N 59 1 1 ?
+E -64 1 0 ?
+M 50 1 0 ?
+R 33 1 0 ?
+A 218 1 0 ?
+F 53 1 1 ?
+S 218 1 0 ?
+K -5 1 0 ?
+M 53 1 1 ?
+T 51 1 0 ?
+I -40 1 0 ?
+I 114 1 1 ?
+G 181 1 0 ?
+K 119 1 0 ?
+F 69 1 1 ?
+W -108 1 0 ?
+D -12 1 0 ?
+D 112 1 0 ?
+A 25 1 0 ?
+E 89 1 1 ?
+S 9 1 0 ?
+N 188 1 0 ?
+D 237 1 1 ?
+V 75 1 0 ?
+E -42 1 0 ?
+H 68 1 1 ?
+V 61 1 0 ?
+D 89 1 0 ?
+D 195 1 0 ?
+K 109 1 1 ?
+L 111 1 0 ?
+S 180 1 1 ?
+M 109 1 0 ?
+I 36 1 0 ?
+C -28 1 0 ?
+E 224 1 0 ?
+V 194 1 0 ?
+I 184 1 1 ?
+I 231 1 0 ?
+H 65 1 0 ?
+T 76 1 0 ?
+H 142 1 0 ?
+T 242 1 1 ?
+A 85 1 0 ?
+E 101 1 0 ?
+E 30 1 1 ?
+G 5 1 1 ?
+Y -15 1 1 ?
+R -88 1 1 ?
+Q -8 1 1 ?
+L 58 1 0 ?
+E -7 1 1 ?
+Y 94 1 0 ?
+T -23 1 0 ?
+K 207 1 0 ?
+P 186 1 0 ?
+F 30 1 1 ?
+G 238 1 1 ?
+E 142 1 0 ?
+E 26 1 0 ?
+I 68 1 0 ?
+E 149 1 1 ?
+R -11 1 0 ?
+S -29 1 0 ?
+T -39 1 0 ?
+R 219 1 0 ?
+V 104 1 0 ?
+I 23 1 0 ?
+T 224 1 0 ?
+N -57 1 0 ?
+L -21 1 0 ?
+M 18 1 1 ?
+K -24 1 1 ?
+D 77 1 1 ?
+L -41 1 0 ?
+R -75 1 0 ?
+Y 37 1 0 ?
+L 227 1 1 ?
+A 34 1 0 ?
+E 41 1 0 ?
+L 23 1 0 ?
+I -31 1 0 ?
+Q 134 1 0 ?
+I 44 1 0 ?
+T 60 1 0 ?
+A -7 1 1 ?
+L 173 1 0 ?
+G 191 1 0 ?
+G 48 1 0 ?
+A 125 1 1 ?
+Q 235 1 1 ?
+T 215 1 1 ?
+G 110 1 0 ?
+V 105 1 0 ?
+A 72 1 0 ?
+W -109 1 1 ?
+D -33 1 0 ?
+Q -60 1 0 ?
+I 209 1 1 ?
+E 167 1 0 ?
+L 207 1 1 ?
+Q -15 1 0 ?
+S -17 1 1 ?
+D 33 1 0 ?
+D -22 1 0 ?
+D 96 1 0 ?
+K 72 1 0 ?
+Q 127 1 0 ?
+K -48 1 0 ?
+Q -28 1 0 ?
+H -71 1 1 ?
+Q 219 1 1 ?
+H -56 1 0 ?
+A 76 1 0 ?
+L 147 1 0 ?
+V 10 1 1 ?
+L 46 1 0 ?
+R 166 1 0 ?
+Y -88 1 0 ?
+G 68 1 0 ?
+E 18 1 1 ?
+D -44 1 0 ?
+V 128 1 0 lysine adduct
+I -43 1 0 ?
+W 227 1 0 ?
+K 124 1 0 ?
+V 150 1 0 ?
+T 20 1 0 ?
+F -4 1 1 ?
+M 33 1 1 ?
+F -31 1 0 ?
+G 18 1 1 ?
+C -54 1 0 ?
+D 74 1 0 ?
+I -16 1 0 ?
+S 77 1 0 ?
+K 42 1 0 acetylation
+Y 183 1 0 ?
+P 156 1 1 ?
+N 17 1 0 ?
+P 138 1 0 ?
+L 7 1 1 ?
+F -29 1 1 ?
+N 200 1 1 ?
+Q 54 1 1 ?
+I 121 1 0 ?
+L 98 1 0 ?
+Q 156 1 0 ?
+Q -36 1 1 ?
+I 116 1 0 ?
+N -48 1 0 ?
+L 8 1 0 ?
+E 239 1 0 ?
+I 117 1 0 ?
+Q 231 1 0 ?
+A 207 1 0 ?
+S 15 1 1 ?
+D -41 1 0 ?
+Y -74 1 0 ?
+R 230 1 0 ?
+L -13 1 0 ?
+F 12 1 0 ?
+A 15 1 1 ?
+Y 64 1 0 ?
+D 94 1 1 ?
+K -9 1 1 ?
+S 17 1 0 ?
+L 241 1 0 ?
+F -85 1 1 ?
+R 161 1 0 ?
+T -30 1 0 ?
+F -18 1 1 ?
+Q -6 1 1 ?
+K 129 1 0 ?
+Y 203 1 0 ?
+E 37 1 0 ?
+G 50 1 1 ?
+L 5 1 1 ?
+C 39 1 0 ?
+R 65 1 1 ?
+I -11 1 0 ?
+A 32 1 0 ?
+F 52 1 0 ?
+I 131 1 0 ?
+K 122 1 0 ?
+I 47 1 0 ?
+Q 114 1 0 ?
+L 56 1 0 ?
+P -9 1 0 ?
+W 183 1 0 ?
+P -32 1 0 ?
+N 211 1 1 ?
+E 140 1 0 ?
+D 50 1 0 ?
+M 56 1 1 ?
+C 216 1 0 ?
+L 148 1 0 ?
+L 205 1 1 ?
+D 82 1 0 ?
+L -38 1 0 ?
+G 31 1 0 ?
+K -3 1 0 ?
+L 175 1 0 ?
+S -13 1 0 ?
+M 215 1 0 ?
+C 8 1 0 ?
+G 222 1 0 ?
+D 170 1 0 ?
+D 153 1 0 ?
+N 178 1 0 ?
+C -59 1 1 ?
+R 168 1 1 ?
+A 170 1 0 ?
+R -49 1 0 ?
+E 62 1 0 ?
+R -98 1 0 ?
+C 54 1 0 ?
+G 137 1 0 ?
+R 103 1 0 ?
+D 8 1 1 ?
+D -24 1 0 ?
+D 23 1 1 ?
+A 111 1 0 ?
+L 156 1 0 ?
+W -58 1 0 ?
+E 79 1 0 ?
+K 8 1 0 ?
+V 222 1 1 ?
+Q -65 1 0 ?
+D 63 1 0 ?
+P 93 1 1 ?
+L 44 1 1 ?
+Y -31 1 0 ?
+H 6 1 0 ?
+D 57 1 0 NT+CAM
+M -11 1 1 ?
+V 161 1 1 ?
+V -27 1 0 ?
+E -72 1 1 ?
+H -15 1 1 ?
+M 85 1 0 ?
+Q 203 1 0 ?
+I -27 1 0 ?
+C 207 1 0 ?
+T 58 1 0 ?
+A 160 1 0 ?
+C 157 1 0 ?
+V 137 1 0 ?
+P 102 1 1 ?
+A 13 1 0 ?
+S -15 1 0 ?
+V 176 1 0 ?
+Q -25 1 0 ?
+R 153 1 0 ?
+Y -9 1 0 ?
+Y -91 1 0 ?
+L 229 1 1 ?
+W 179 1 1 ?
+D 40 1 0 ?
+C 111 1 0 ?
+D 211 1 0 ?
+A 157 1 1 ?
+E 157 1 0 ?
+M 200 1 0 ?
+S 3 1 1 ?
+F -33 1 1 ?
+P -15 1 1 ?
+T 197 1 0 ?
+E 72 1 0 ?
+N 14 1 1 ?
+R 215 1 0 ?
+Q 160 1 0 ?
+C -25 1 1 ?
+F 91 1 0 ?
+A 230 1 0 ?
+P 66 1 0 ?
+W 5 1 1 ?
+S 110